Kpn AI, a new type I restriction-modification system in Klebsiella pneumoniae 1 1Edited by K.Yamamoto
1997; Elsevier BV; Volume: 271; Issue: 3 Linguagem: Inglês
10.1006/jmbi.1997.1202
ISSN1089-8638
AutoresNan Sook Lee, Obed Rutebuka, Takeshi Arakawa, Thomas A. Bickle, Junichi Ryu,
Tópico(s)Bacterial Genetics and Biotechnology
ResumoThe KpnAI restriction-modification (R-M) system has been identified in Klebsiella pneumoniae strain M5a1. The restriction gene of KpnAI was first cloned into pBR322 using an r−m+ M5a1 derivative and phage SBS for screening. Subsequently, an adjacent DNA fragment showing modification activity was cloned into pUC19. A total of 7.2 kb DNA sequencing data revealed three open reading frames, corresponding to hsdR, hsdM and hsdS genes of type I R-M systems. The predicted hsdR, hsdM and hsdS-coded peptides shared 95%, 98% and 44% identity, respectively, with the corresponding peptides of the recently identified StySBLI system, a prototype of the type ID family. This high homology suggests thatKpnAI is also a member of the type ID family. The KpnAI system seems to be the first type I system identified in Klebsiella species.
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