Isolation and characterisation of the cell-wall fibres of carrot
1988; Elsevier BV; Volume: 172; Issue: 2 Linguagem: Inglês
10.1016/s0008-6215(00)90856-5
ISSN1873-426X
AutoresP. Massiot, Xavier Rouau, Jean‐François Thibault,
Tópico(s)Postharvest Quality and Shelf Life Management
ResumoCell-wall fractions have been prepared from an alcohol-insoluble-residue of carrot root by treatment with (a) Pronase to remove the cytoplasmic proteins, (b) hot dilute acid and cold dilute alkali to give pectin-free residues, and (c) concentrated alkali to leave the α-cellulose and lignin. The purified cell-wall material still contained ∼ 1% protein and was composed mainly of cellulose, lignin, methyl-esterified galacturonic acid, and smaller amounts of galactose and arabinose. Methylation analysis of the insoluble residues indicated the presence, in order of decreasing concentration, of rhamnogalacturonan with the rhamnosyl residues carrying side chains at position 4, cellulose, (1→4)-linked galactan, (1→5)-linked arabinan, (1→4)-linked xylan, (1→4)-linked mannan, and xyloglucan.
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