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Analyses of pesticide residues in fruit‐based baby food by liquid chromatography/electrospray ionization time‐of‐flight mass spectrometry

2007; Wiley; Volume: 21; Issue: 13 Linguagem: Inglês

10.1002/rcm.3062

1097-0231

Bienvenida Gilbert‐López, Juan F. García‐Reyes, P. Ortega‐Barrales, Antonio Molina‐Díaz, Amadeo R. Fernández‐Alba,

Pharmacological Effects and Assays

Abstract A liquid chromatography/electrospray ionization time‐of‐flight mass spectrometry (LC/ESI‐TOFMS) method has been developed for the determination of 12 pesticides (namely, carbendazim, thiabendazole, imazalil, tridemorph, triadimefon, bitertanol, prochloraz, flutriafol, myclobutanil, iprodione, diphenylamine and procymidone) in fruit‐based baby food (multi‐fruit jars and juices intended for infant consumption). The developed method consists of a sample treatment step based on liquid‐liquid extraction using acetonitrile, followed by a clean‐up step based on dispersive solid‐phase extraction (SPE) with a primary‐secondary amine (PSA). Multi‐fruit and apple juices were processed by a SPE procedure using Oasis HLB™ cartridges. Subsequent identification and quantitation was accomplished by LC/ESI‐TOFMS analysis: the confirmation of the target pesticides was based on accurate mass measurements of selected ions (protonated molecules ([M+H] + ) and fragment ions). Confirmation studies were accomplished at low concentration levels (10 µg kg −1 ) and accuracy errors lower than 2 ppm were obtained in most cases. Baby food extracts spiked at 10 µg kg −1 fortification level yielded average recoveries in the range 78–105% with relative standard deviations less than 10% for most of the analytes. Limits of detection (LODs) were between 0.1 and 4 µg kg −1 depending on the pesticide studied. Finally, the proposed method was applied to a total of 33 baby food samples from Spain and the United Kingdom. Although imazalil, thiabendazole and carbendazim were detected in a high number – over 60%– of baby food samples, none of the samples tested were found to be above the 0.01 mg kg −1 EU standard. Copyright © 2007 John Wiley & Sons, Ltd.