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Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii , and their cross‐species utilization

2007; Wiley; Volume: 8; Issue: 1 Linguagem: Inglês

10.1111/j.1471-8286.2007.01913.x

1755-0998

Britta Denise Hardesty, Sara Hughes, V. M. RODRIGUEZ, Julie A. Hawkins,

Botanical Research and Applications

Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty-seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.