The effect of a purified α-L-arabinofuranosidase on some arabinose-containing polysaccharides
1968; Elsevier BV; Volume: 8; Issue: 1 Linguagem: Inglês
10.1016/s0008-6215(00)81696-1
ISSN1873-426X
Autores Tópico(s)Carbohydrate Chemistry and Synthesis
Resumoα-L-Arabinofuranosidase catalyzes the hydrolysis of nonreducing terminal α-L-arabinofuranosidic linkages in L-arabinan, arabinoxylan, and other Larabinose-containing polysaccharides. In Aspergillus niger, this enzyme is produced inducibly in a medium containing L-arabinose or Larabinan. Phenyl- or p-nitrophenyl-α-L-arabinofuranoside is hydrolyzed by this enzyme action, thus, the amount of reducing group or nittrophenolate ion liberated is a measure of the enzyme activity. This chapter describes the colorimetoric assay method using p-nitrophenyl-α-L-arabinofuranoside. The chapter discusses the preparation and purification procedure of α-L-arabinofuranosidase from Aspergillus niger. The chapter concludes with a discussion on the physicochemical properties, stability, and substrate specificity of the enzyme. The enzyme is highly specific for nonreducing terminal α-L-arabinofuranosidic linkages and will not attack internal a-L-arabinofuranosyl linkages.
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