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Growth of herpes simplex virus in epidermal keratinocytes determines cutaneous pathogenicity in mice

2005; Wiley; Volume: 75; Issue: 3 Linguagem: Inglês

10.1002/jmv.20284

1096-9071

Yoshihiro Yoshida, Zhihong Li, Masahiko Kurokawa, Takashi Kawana, Masami Imakita, Kimíyasu Shiraki,

Contact Dermatitis and Allergies

Journal of Medical VirologyVolume 75, Issue 3 p. 421-426 Growth of herpes simplex virus in epidermal keratinocytes determines cutaneous pathogenicity in mice Yoshihiro Yoshida, Yoshihiro Yoshida Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorZhiHong Li, ZhiHong Li Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorMasahiko Kurokawa, Masahiko Kurokawa Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorTakashi Kawana, Takashi Kawana Department of Gynecology, Teikyo University, Tokyo, JapanSearch for more papers by this authorMasami Imakita, Masami Imakita Department of Pathology, Rinku General Medical Center, Izumisano Municipal Hospital, Osaka, JapanSearch for more papers by this authorKimiyasu Shiraki, Corresponding Author Kimiyasu Shiraki kshiraki@toyama-mpu.ac.jp Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanProfessor and Chairman, Department of Virology, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.===Search for more papers by this author Yoshihiro Yoshida, Yoshihiro Yoshida Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorZhiHong Li, ZhiHong Li Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorMasahiko Kurokawa, Masahiko Kurokawa Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanSearch for more papers by this authorTakashi Kawana, Takashi Kawana Department of Gynecology, Teikyo University, Tokyo, JapanSearch for more papers by this authorMasami Imakita, Masami Imakita Department of Pathology, Rinku General Medical Center, Izumisano Municipal Hospital, Osaka, JapanSearch for more papers by this authorKimiyasu Shiraki, Corresponding Author Kimiyasu Shiraki kshiraki@toyama-mpu.ac.jp Department of Virology, Toyama Medical and Pharmaceutical University, Toyama, JapanProfessor and Chairman, Department of Virology, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.===Search for more papers by this author First published: 12 January 2005 https://doi.org/10.1002/jmv.20284Citations: 9AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Herpes simplex viruses (HSV)-1 and -2 isolated from genital lesions were examined for cutaneous pathogenicity and its correlation with cellular tropism. HSV-1 caused vesiculation, erosion/ulcer, and zosteriform lesions successively, but skin lesions of HSV-2 developed without vesiculation in some mice, and with statistically significantly less frequent vesiculation than HSV-1. Thus, the virological type of HSV was correlated with its cutaneous pathogenicity. The growth characteristics of HSV-1 and -2 were compared in cultured human embryonic lung (HEL) fibroblasts, human lung cancer A549 cells, human neonatal epidermal keratinocytes, human neonatal dermal fibroblasts, HeLa cells, and Vero cells. HSV-2 produced plaques that were 72% times the size of HSV-1 plaques in epidermal keratinocytes but 230%–500% the size in the other cells. The difference between HSV-1 and -2 in the ratio of plaque size to virus yield in epidermal keratinocytes was much larger (502 times) than the ratio of the other cells (5.57–28.8 times). Keratinocytes are the major constituent of the epidermal layer of the skin and the cells in which vesiculation and erosion/ulceration occur histologically. Therefore, the smaller spread of HSV-2 in keratinocytes of the epidermal layer and the greater spread in other cells of the dermal layer might reflect its lesser invasiveness in the epidermal layer despite larger invasiveness in the dermal layer, which is reflected in the low incidence of erosion/ulcer of the skin compared to HSV-1. Thus, the growth of HSV in epidermal keratinocytes appeared to correlate with the cutaneous pathogenicity causing vesiculation in the skin. J. Med. Virol. 75:421–426, 2005. © 2005 Wiley-Liss, Inc. Citing Literature Volume75, Issue3March 2005Pages 421-426 RelatedInformation