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Cultured Human Keratinocytes Express Tropoelastin

1997; Elsevier BV; Volume: 109; Issue: 5 Linguagem: Inglês

10.1111/1523-1747.ep12337639

1523-1747

Hiroyoshi Kajiya, Nobuhiko Tanaka, Toyoko Inazumi, Yoshiyuki Seyama, Shingo Tajima, Akira Ishibashi,

Protease and Inhibitor Mechanisms

We detected elastin mRNA in cultured normal human keratinocytes by RNase protection assay. The content of elastin mRNA was estimated at approximately one-twentieth of that of cultured skin fibroblasts. Tropoelastin polypeptide with a molecular weight of 68 kDa was detected in the preparation of culture medium of normal human keratinocytes by western blot assays using anti-tropoelastin antibody. Immunohistochemical studies also demonstrated positive staining in cultured normal human keratinocytes as well as in skin fibroblasts. The expression of elastin by normal human keratinocytes was found to reach a maximum level at the quiescent phase of keratinocyte growth. When normal human keratinocytes were cultured on tropoelastin-coated dishes, their growth potential was greatly suppressed compared with other matrix protein-coated dishes. These results suggest that cultured normal human keratinocytes can actively synthesize elastin and that keratinocyte elastin may act as a growth-regulator for keratinocytes.