Portal de Periódicos da CAPES

Mutagenicity of commercial p-phenylenediamine and of an oxidation mixture of p-phenylenediamine and resorcinol in salmonella typhimurium TA98

1981; Elsevier BV; Volume: 19; Linguagem: Inglês

10.1016/0015-6264(81)90307-2

1878-6049

Riccardo Crebelli, L. Conti, A. Carere, Romano Zito,

DNA and Nucleic Acid Chemistry

One chemically purified sample of p-phenylenediamine and two commercial analytical-grade samples, as well as an oxidation mixture prepared by reaction of p-phenylenediamine and resorcinol with H2O2, were tested for their ability to revert the frameshift strain TA98 in the Salmonella typhimurium/microsomal assay. While the purified product was non-mutagenic in this system, the two commercial samples of p-phenylenediamine and the oxidation mixture were mutagenic in the presence of S-9 mix. Separation and purification of four major fractions of the oxidation mixture indicated that only one oxidation product of p-phenylenediamine and resorcinol was responsible for the mutagenic activity observed. When rats were treated topically with the oxidation mixture, their urine became mutagenic. This activity was apparently attributable to the previously isolated compound, which was found in the urine in acetylated form.