Phosphorylation of PERIOD Is Influenced by Cycling Physical Associations of DOUBLE-TIME, PERIOD, and TIMELESS in the Drosophila Clock
2001; Cell Press; Volume: 30; Issue: 3 Linguagem: Inglês
10.1016/s0896-6273(01)00320-8
ISSN1097-4199
AutoresBrian Kloss, Adrian Rothenfluh, Michael W. Young, Lino Sáez,
Tópico(s)Sleep and Wakefulness Research
ResumoThe clock gene double-time (dbt) encodes an ortholog of casein kinase Iϵ that promotes phosphorylation and turnover of the PERIOD protein. Whereas the period (per), timeless (tim), and dClock (dClk) genes of Drosophila each contribute cycling mRNA and protein to a circadian clock, dbt RNA and DBT protein are constitutively expressed. Robust circadian changes in DBT subcellular localization are nevertheless observed in clock-containing cells of the fly head. These localization rhythms accompany formation of protein complexes that include PER, TIM, and DBT, and reflect periodic redistribution between the nucleus and the cytoplasm. Nuclear phosphorylation of PER is strongly enhanced when TIM is removed from PER/TIM/DBT complexes. The varying associations of PER, DBT and TIM appear to determine the onset and duration of nuclear PER function within the Drosophila clock.
Referência(s)