Albumin synthesis by liver parenchymal cells isolated from young, adult and old rats
1976; Elsevier BV; Volume: 71; Issue: 2 Linguagem: Inglês
10.1016/0006-291x(76)90817-2
ISSN1090-2104
AutoresC.F.A. van Bezooijen, Tsehai A.J. Grell, D.L. Knook,
Tópico(s)Chromatography in Natural Products
ResumoViable isolated parenchymal cells were incubated in a modified Waymouth medium under an oxygen tension of 30×103 Pa at pH 7.8. Under these conditions, hepatocytes from 3-month-old rats synthesized 5.8 μg albumin/h/106 cells. This value nearly equals the synthesizing capacity of intact liver tissue and is the highest activity reported so far for isolated hepatocytes. Parenchymal cells isolated from 36-month-old rats synthesized more albumin as compared to cells from 3-month-old rats. The albumin synthesizing capacity of cells isolated from 12-month-old rats was less than that of cells from 3-month-old rats.
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