Adaptation of Lymphadenopathy Associated Virus (LAV) to Replication in EBV-Transformed B Lymphoblastoid Cell Lines
1984; American Association for the Advancement of Science; Volume: 225; Issue: 4657 Linguagem: Inglês
10.1126/science.6328661
ISSN1095-9203
AutoresLuc Montagnier, J Gruest, S. Chamaret, C. Dauguet, C. Axler, Denise Guétard, M.T. Nugeyre, Françoise Barré‐Sinoussi, Jean‐Claude Chermann, Jean‐Baptiste Brunet, David Klatzmann, J. C. Gluckman,
Tópico(s)Viral-associated cancers and disorders
ResumoA strain of lymphadenopathy associated retrovirus (LAV) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor. The virus produced from this line (B-LAV) was also able to grow at a high rate in some other lymphoblastoid lines and in a Burkitt lymphoma line. This adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism for normal T4 + lymphocytes. It is thus possible to grow LAV in large quantities that can be used for the preparation of diagnostic reagents. The interaction between such a human retrovirus and Epstein-Barr virus, a DNA virus, may have some implication for the pathology of the acquired immunodeficiency syndrome and related diseases.
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