The sperm storage in the seminal receptacle in cochlostoma montanum (issel) (gastropoda prosobranchia)
1983; Elsevier BV; Volume: 12; Issue: 1-2 Linguagem: Inglês
10.1016/0304-3991(83)90408-4
ISSN1879-2723
AutoresMaria Gloria Selmi, Folco Giusti,
Tópico(s)Marine Biology and Environmental Chemistry
ResumoProthylakoids (PT's) were separated from prolamellar bodies (PLB's) of etioplasts obtained from leaves of Avena seedlings grown in complete darkness of after 3–5 min. illumination. In part of the leaves protochlorophyll (ide) content was increased by treatment with δ-aminolevulinic acid (ALA) prior to the isolation of the etioplasts. Protochlorophyl(ide), chlorophyll (ide) and saponin content (the latter as an indication for presence of tubules from the PLB's) of the fractions was determined. In the isolated PLB and PT fractions about 75 % of the saponin content of the «broken etioplast» (BE) fraction could be recovered. The PT fraction contained only 4.6 % of the saponins, indicating a high degree of purity. The PLB fraction was enriched in PLB's, but contained an unknown amount of PT's.About 80 % of the chlorophyllous pigments in the BE fraction were recovered in the PT and PLB fractions independent of light and/or ALA treatment of the plants. Thus, both endogenous PChl(ide) as well as the ALA-induced PChl(ide) are bound to membranes which can be sedimented at relatively low g-values.About 1/3 of the endogenous PChl(ide) and Chl(ide) and of the ALA-induced PChl(ide) was found consistently in the PT fraction, independent of light treatment. Since the ratio chlorophlls/saponin in the PT's was about 6 times higher in the PT fraction than in the PLB fraction it is evident that in Avena etioplasts the pigment content of the PT membrane is much higher than that of the PLB tubules. The possibility is discussed that most of the chlorophyll may be located in the prothylakoids only.
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