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Characterization and enzymatic synthesis of acyl galactosyl monoglyceride

1973; Elsevier BV; Volume: 326; Issue: 2 Linguagem: Inglês

10.1016/0005-2760(73)90244-0

1879-145X

Christa Critchley, Ernst Heinz,

Metabolism and Genetic Disorders

An unknown lipid was isolated from leaf homogenates. The glycolipid is slightly more hydrophobic than monogalactosyi diglyceride, but composed likewise of giycerol, galactose and fatty acids in a molar ratio of 1:1:2. By NMR and mass spectroscopy the structure was determined to be 1′-O-acyl-3′-O-(6-O-acyl-β-d-galactopyranosyl) -sn-glycerol. For comparison this compound has also been prepared semisynthetically. Enzymatic experiments with radioactive substrates showed that in vitro acyl galactosyl monoglyceride can originate from monogalactosyl monoglyceride by accepting an additional fatty acid, which is derived either from another molecule of monogalactosyl monoglyceride or from digalactosyl diglyceride. These reactions resemble those involved in the formation of acyl galactosyl diglyceride.