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HPLC Method for Detection of Ergotamine, Ergosine, and Ergine after Intravenous Injection of a Single Dose

1996; American Chemical Society; Volume: 44; Issue: 1 Linguagem: Inglês

10.1021/jf950425i

1520-5118

Ali S. Moubarak, E. L. Piper, Z. B. Johnson, Miroslav Flieger,

Nematode management and characterization studies

A simple, rapid method of liquid solid extraction coupled with a sensitive HPLC procedure with fluorescence detection to measure ergot alkaloid levels in bovine blood samples is presented. Single injections of either ergosine, ergotamine, or ergine were given to Holstein steer calves (140 kg), and serum concentrations of the ergot alkaloids were monitored over a 2-h period. Serum samples were loaded on a Bond Elut (100 mg) C18 column that had been washed with methanol and distilled water. Ergot alkaloids were then eluted with methanol and analyzed by HPLC. Recovery of ergosine, when added to serum at concentrations of 0.5−24.0 ng/mL, ranged from 95 to 98%. The data fit previous reports of three phases in elimination of drug. The method reported has excellent repeatability, a detection limit of 0.5 ng/mL, and provides an alternative to quantitate ergosine, ergotamine, and ergine in bovine blood samples at therapeutic dosages. Keywords: Ergosine; ergotamine; ergine; blood; assay; HPLC