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A Fusarium sp. different from Fusarium oxysporum and F. mangiferae is associated with mango malformation in Michoacán, Mexico

2008; Wiley; Volume: 57; Issue: 4 Linguagem: Inglês

10.1111/j.1365-3059.2007.01811.x

1365-3059

Gerardo Rodríguez‐Alvarado, Sylvia Patricia Fernández Pavía, R. C. Ploetz, M. Valenzuela‐Vázquez,

Plant-Microbe Interactions and Immunity

Plant PathologyVolume 57, Issue 4 p. 781-781 Free Access A Fusarium sp. different from Fusarium oxysporum and F. mangiferae is associated with mango malformation in Michoacán, Mexico G. Rodríguez-Alvarado, Corresponding Author G. Rodríguez-Alvarado Laboratorio de Patología Vegetal, IIAF-UMSNH, Km. 9.5 Car. Morelia-Zinapecuaro, Michoacán 58880, México; *E-mail: [email protected]Search for more papers by this authorS. P. Fernández-Pavía, S. P. Fernández-Pavía Laboratorio de Patología Vegetal, IIAF-UMSNH, Km. 9.5 Car. Morelia-Zinapecuaro, Michoacán 58880, México;Search for more papers by this authorR. C. Ploetz, R. C. Ploetz University of Florida, Department of Plant Pathology, Tropical Research and Education Center, Homestead 33031, USA; andSearch for more papers by this authorM. Valenzuela-Vázquez, M. Valenzuela-Vázquez Universidad Autónoma de Ciudad Juárez, Cd. Juárez, Chihuahua 32300, MéxicoSearch for more papers by this author G. Rodríguez-Alvarado, Corresponding Author G. Rodríguez-Alvarado Laboratorio de Patología Vegetal, IIAF-UMSNH, Km. 9.5 Car. Morelia-Zinapecuaro, Michoacán 58880, México; *E-mail: [email protected]Search for more papers by this authorS. P. Fernández-Pavía, S. P. Fernández-Pavía Laboratorio de Patología Vegetal, IIAF-UMSNH, Km. 9.5 Car. Morelia-Zinapecuaro, Michoacán 58880, México;Search for more papers by this authorR. C. Ploetz, R. C. Ploetz University of Florida, Department of Plant Pathology, Tropical Research and Education Center, Homestead 33031, USA; andSearch for more papers by this authorM. Valenzuela-Vázquez, M. Valenzuela-Vázquez Universidad Autónoma de Ciudad Juárez, Cd. Juárez, Chihuahua 32300, MéxicoSearch for more papers by this author First published: 18 July 2008 https://doi.org/10.1111/j.1365-3059.2007.01811.xCitations: 16AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Malformation of mango has a pantropical distribution. In 1958, it was first reported in México where it now limits production in several southern states. Fusarium oxysporum, F. proliferatum and F. sterilihyphosum have been associated with the disease worldwide, but Koch's postulates have been completed only with F. mangiferae (Marasas et al., 2006). A Fusarium sp. was recovered in 2004 and 2005 in Michoacán, México from 27 mango trees with typical symptoms of malformation. All 147 isolates differed from F. oxysporum, F. proliferatum and F. mangiferae, none of which were found in affected trees. Fourteen representative, single-spored isolates from eight production areas were dark pink to salmon-coloured on potato dextrose agar. On carnation leaf agar chlamydospores were absent, three- to five-celled macroconidia rare (29–42 µm long), but microconidia copious on mono- and polyphialides. Of the above taxa, only F. oxysporum produces chlamydospores, only F. proliferatum, F. sterilihyphosum, and F. mangiferae produce polyphialides, and only F. sterilihyphosum produces coils of sterile hyphae. A F. mangiferae–diagnostic PCR primer pair (1-3F/R) did not amplify DNA from the Michoacán strains, but a primer pair that amplifies DNA from F. sterilihyphosum but not F. mangiferae (61-2F/R) amplified the reported 445 bp-fragment (Zheng & Ploetz, 2002). The Michoacán strains grew more than 50% faster than F. sterilihyphosum (Rodriguez-Alvarado et al., 2006), and possessed a 625 bp sequence of the translation elongation factor-1α that differed at 18 nucleotide positions from a F. sterilihyphosum sequence in GenBank (Accession No. DQ452858·1). Malformation symptoms were produced after 12 weeks on nucellar, 9-month-old 'Ataulfo' mango seedlings inoculated separately with four isolates of the Fusarium sp. using standard protocols (Freeman et al., 1999), but not on water-inoculated control plants. Inoculated strains were re-isolated. It is clear that a taxon other than F. mangiferae causes mango malformation in Michoacán. Our findings challenge previous reports that F. oxysporum causes mango malformation in Michoacán, though isolates from mango were not available. Further work is also needed to compare this Fusarium sp. and F. sterilihyphosum. References Freeman S, Maimon M, Pinkas Y, 1999. Use of GUS transformants of Fusarium subglutinans for determining etiology of mango malformation disease. Phytopathology 89, 456– 61. Marasas WFO, Ploetz RC, Wingfield MJ, Wingfield BD, Steenkamp ET, 2006. Mango malformation disease and the associated Fusarium species. Phytopathology 96, 667– 72. Rodriguez-Alvarado G, Fernández-Pavía SP, Ploetz RC, 2006. Fusarium sp. characterization causing mango malformation in Michoacan Mexico. Phytopathology 96, S99. Zheng Q, Ploetz RC, 2002. Genetic diversity in the mango malformation pathogen and development of a PCR assay. Plant Pathology 51, 208– 16. Citing Literature Volume57, Issue4August 2008Pages 781-781 ReferencesRelatedInformation