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RNA-seq analysis of prostate cancer in the Chinese population identifies recurrent gene fusions, cancer-associated long noncoding RNAs and aberrant alternative splicings

2012; Springer Nature; Volume: 22; Issue: 5 Linguagem: Inglês

10.1038/cr.2012.30

1748-7838

Shancheng Ren, Zhiyu Peng, Jian‐Hua Mao, Yongwei Yu, Changjun Yin, Xin Gao, Zilian Cui, Jibin Zhang, Yi Kang, Weidong Xu, Chao Chen, Fubo Wang, Xinwu Guo, Ji Lu, Jun Yang, Min Wei, Zhijian Tian, Yinghui Guan, Liang Tang, Chuanliang Xu, Linhui Wang, Xu Gao, Wei Tian, Jian Wang, Huanming Yang, Jun Wang, Yinghao Sun,

Prostate Cancer Treatment and Research

There are remarkable disparities among patients of different races with prostate cancer; however, the mechanism underlying this difference remains unclear. Here, we present a comprehensive landscape of the transcriptome profiles of 14 primary prostate cancers and their paired normal counterparts from the Chinese population using RNA-seq, revealing tremendous diversity across prostate cancer transcriptomes with respect to gene fusions, long noncoding RNAs (long ncRNA), alternative splicing and somatic mutations. Three of the 14 tumors (21.4%) harbored a TMPRSS2-ERG fusion, and the low prevalence of this fusion in Chinese patients was further confirmed in an additional tumor set (10/54=18.5%). Notably, two novel gene fusions, CTAGE5-KHDRBS3 (20/54=37%) and USP9Y-TTTY15 (19/54=35.2%), occurred frequently in our patient cohort. Further systematic transcriptional profiling identified numerous long ncRNAs that were differentially expressed in the tumors. An analysis of the correlation between expression of long ncRNA and genes suggested that long ncRNAs may have functions beyond transcriptional regulation. This study yielded new insights into the pathogenesis of prostate cancer in the Chinese population.