New high-performance liquid chromatographic method for the determination of alprazolam and its metabolites in serum: instability of 4-hydroxyalprazolam
1991; Elsevier BV; Volume: 568; Issue: 1 Linguagem: Inglês
10.1016/0378-4347(91)80361-f
ISSN1872-812X
AutoresVirginia D. Schmith, Steven R. Cox, Michael A. Zemaitis, Patricia D. Kroboth,
Tópico(s)Antibiotics Pharmacokinetics and Efficacy
ResumoA high-performance liquid chromatographic method was developed for the determination of alprazolam (ALP) and its active metabolites, alpha-hydroxyalprazolam (AOH) and 4-hydroxyalprazolam (4OH) in human serum. During assay development, the instability of 4OH was revealed. Factors affecting stability of 4OH were then investigated. In this report, the assay methodology for the determination of ALP and AOH, the instability of 4OH, subsequent interference of 4OH breakdown products with AOH quantification, and factors affecting 4OH stability are described. The clinical significance of our findings are reported.
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