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Respiration dependent transport of proline by electron transport particles from Mycobacterium phlei

1971; Elsevier BV; Volume: 44; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(71)90609-7

1090-2104

Hajime Hirata, Akira Asano, Arnold F. Brodie,

RNA modifications and cancer

Respiration dependent transport of proline was demonstrated in electron transport particles from Mycobacterium phlei. The uptake proceeded against the concentration gradient with succinate, generated NADH, exogenous NADH or ascorbate-TPD as substrate. The latter two electron donors were most effective for the transport of proline and for oxidation, but they were least efficient for oxidative phosphorylation. Glucose, Dlactate, fumarate and ATP had no effect on the transport process. The transport of proline was sensitive to certain uncoupling agents and respiratory inhibitors. It was independent of oxidative phosphorylation since it proceeded in the absence of coupling factors or phosphate and was not inhibited by arsenate. The transport of proline appears to occur with membranes orientated as in the intact cell.