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In Vitro Skin Penetration of Monoethanolamine and Diethanolamine Using Excised Skin from Rats, Mice, Rabbits, and Humans

1996; Informa; Volume: 15; Issue: 2 Linguagem: Inglês

10.3109/15569529609048869

1532-2505

James D. Sun, Joyce L. Beskitt, Marilyn J. Tallant, Stephen W. Frantz,

Dermatology and Skin Diseases

Monoethanolamine (MEA; CAS No. 141-43-5) and diethanolamine (DEA; CAS No. 111-42-2) are used in a number of cosmetic formulations. Therefore, dermal absorption is an important route of potential human exposure to these compounds. Because of this, the skin penetration characteristics of these two alkanolamines were evaluated using an established in vitro technique. Full-thickness skin preparations from female CD rats, CD-1 mice, New Zealand White rabbits, and female mammoplasty patients were used. Using a dynamic, flow-through design, skin penetration apparatus, undiluted and aqueous solutions of [14C]MEA and [14C]DEA were applied to skin preparations from each species in an “infinite dose” manner at target doses of 4 mg/cm2 and 20 mg/cm2respectively. The time course of 14C penetration was then measured for 6 h. The results showed that undiluted MEA penetrated animal skin better than did undiluted DEA, but was approximately the same for human skin. For the aqueous applications of MEA and DEA, the penetration was similar to each species, except for rabbit skin, in which the permeability appeared to be less for aqueous MEA than it was for aqueous DEA. Comparing undiluted and water diluted doses of each compound, the results showed that there was generally less skin penetration of the undiluted material than that for the diluted test substances. Therefore, the total absorbed dose of MEA or DEA would be less for cutaneous exposures to undiluted MEA or DEA as compared to similar exposures to water solutions of either compound. A comparison of permeability constants among the species tested suggests that the general rank order of skin penetration for both chemicals, undiluted or diluted, as mouse > rabbit > rat > human skin. Therefore, the results from this in vitro study suggest that the potential percutaneous absorption of MEA or DEA would be less for humans than it would be for rats, rabbits, and mice.