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Characterization of digoxigenin-labeled B-phycoerythrin by capillary electrophoresis with laser-induced fluorescence Application to homogeneous digoxin immunoassay

1994; Elsevier BV; Volume: 680; Issue: 2 Linguagem: Inglês

10.1016/0021-9673(94)85139-5

1873-3778

Fu-Tai A. Chen, Stephen L. Pentoney,

Analytical Chemistry and Chromatography

A laser-induced fluorescence (LIF) immunoassay technique based on capillary electrophoretic (CE) separation is demonstrated. The analysis of digoxin in serum at clinically useful concentration levels of 10−9 to 10−10M is achieved using this technique. The chemistry presented here using digoxigenin-labeled B-phycoerythrin was selected as a convenient model for the exploration of CE-LIF-based immunoassays. The LIF system described here exhibits detection limits in the low 10−11M range for several common fluorophores. The data presented in this report are one of the first examples of nanomolar quantitative analysis in a human serum matrix by CE.