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BIBTEX RIS

Immunization with cytoplasmic repetitive antigen and flagellar repetitive antigen of Trypanosoma cruzi stimulates a cellular immune response in mice

2004; Cambridge University Press; Volume: 129; Issue: 5 Linguagem: Inglês

10.1017/s0031182004006043

1469-8161

Valéria Rêgo Alves Pereira, Virgínia Maria Barros de Lorena, Ana Paula Galvão da Silva, E. M. Coutinho, Ester de Melo e Silva, Antonio Ferreira, Paulo J. Miranda, Marco Aurélio Krieger, Samuel Goldenberg, Milena Botelho Pereira Soares, Rodrigo Corrêa‐Oliveira, Yara M. Gomes,

Toxoplasma gondii Research Studies

In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-γ, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4 + T cells were the major source of IFN-γ in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.