Type 1 pilus-mediated bacterial invasion of bladder epithelial cells
2000; Springer Nature; Volume: 19; Issue: 12 Linguagem: Inglês
10.1093/emboj/19.12.2803
ISSN1460-2075
Autores Tópico(s)Escherichia coli research studies
ResumoArticle15 June 2000free access Type 1 pilus-mediated bacterial invasion of bladder epithelial cells Juan J. Martinez Juan J. Martinez Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Matthew A. Mulvey Matthew A. Mulvey Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Joel D. Schilling Joel D. Schilling Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Jerome S. Pinkner Jerome S. Pinkner Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Scott J. Hultgren Corresponding Author Scott J. Hultgren Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Juan J. Martinez Juan J. Martinez Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Matthew A. Mulvey Matthew A. Mulvey Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Joel D. Schilling Joel D. Schilling Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Jerome S. Pinkner Jerome S. Pinkner Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Scott J. Hultgren Corresponding Author Scott J. Hultgren Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA Search for more papers by this author Author Information Juan J. Martinez1, Matthew A. Mulvey1, Joel D. Schilling1, Jerome S. Pinkner1 and Scott J. Hultgren 1 1Department of Molecular Microbiology and Microbial Pathogenesis, Box 8230, Washington University School of Medicine, 660 S Euclid Avenue, St Louis, MO, 63110 USA ‡J.J.Martinez and M.A.Mulvey contributed equally to this work *Corresponding author. E-mail: [email protected] The EMBO Journal (2000)19:2803-2812https://doi.org/10.1093/emboj/19.12.2803 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Most strains of uropathogenic Escherichia coli (UPEC) encode filamentous adhesive organelles called type 1 pili. We have determined that the type 1 pilus adhesin, FimH, mediates not only bacterial adherence, but also invasion of human bladder epithelial cells. In contrast, adherence mediated by another pilus adhesin, PapG, did not initiate bacterial internalization. FimH-mediated invasion required localized host actin reorganization, phosphoinositide 3-kinase (PI 3-kinase) activation and host protein tyrosine phosphorylation, but not activation of Src-family tyrosine kinases. Phosphorylation of focal adhesin kinase (FAK) at Tyr397 and the formation of complexes between FAK and PI 3-kinase and between α-actinin and vinculin were found to correlate with type 1 pilus-mediated bacterial invasion. Inhibitors that prevented bacterial invasion also blocked the formation of these complexes. Our results demonstrate that UPEC strains are not strictly extracellular pathogens and that the type 1 pilus adhesin FimH can directly trigger host cell signaling cascades that lead to bacterial internalization. Introduction Urinary tract infections (UTIs) account for an estimated 6–7 × 106 out-patient hospital visits per year and are associated with considerable morbidity (Barnett and Stevens, 1997; Hooton and Stamm, 1997). Escherichia coli is the etiological agent in >80% of all UTIs. The vast majority of E.coli UTI isolates encode 1–2 μm long filamentous surface-adhesive organelles called type 1 pili (Langermann et al., 1997). These organelles are composite fibers consisting of a 7 nm thick helical rod made up of repeating FimA subunits joined to a 3 nm wide distal tip fibrillum structure containing two adaptor proteins, FimF and FimG, and the adhesin, FimH (Russell and Orndorff, 1992; Jones et al., 1995). FimH binds mannose-containing glycoprotein receptors and can mediate bacterial attachment to a variety of different host cell types (Tewari et al., 1993; Baorto et al., 1997; Malaviya and Abraham, 1998; Mulvey et al., 1998). Interactions between FimH and receptors expressed on the lumenal surface of the bladder epithelium appear to be critical to the ability of many uropathogenic E.coli (UPEC) strains to colonize the bladder and cause disease (Connell et al., 1996; Langermann et al., 1997; Thankavel et al., 1997; Mulvey et al., 1998). Subsequent to attachment, it is generally considered that UPEC strains exist as extracellular pathogens within the urinary tract. More than a decade ago, however, transmission electron microscopy (TEM) studies of infected rat and mouse bladders indicated that bladder epithelial cells could internalize UPEC in vivo (Fukushi et al., 1979; McTaggart et al., 1990). Bacteria were observed within membrane-bound vacuoles and free within the cytoplasm of the superficial epithelial cells that line the lumenal surface of the bladder. It was proposed that the bladder epithelial cells internalized bacteria as part of an innate host defense mechanism. A more recent study, however, suggested that bacterial internalization by bladder epithelial cells could benefit the pathogens (Mulvey et al., 1998). Using a murine cystitis model, it was shown that a subpopulation of type 1-piliated E.coli inoculated into the bladder could invade the epithelium and that these bacteria appeared to have a distinct survival advantage over their extracellular counterparts. In general, invasion of host cells by pathogenic bacteria can allow pathogens to evade host defenses and facilitate their dissemination both within and across cellular barriers (Sansonetti, 1991; Falkow et al., 1992; Menard et al., 1996). Invasion of bladder epithelial cells by UPEC may allow these bacteria to avoid the bulk flow of urine through the bladder and may give them access to a more nutrient-rich environment. Intracellular uropathogens may also be better able to avoid the diverse array of antimicrobial substances and immune cells present within the urine and the bladder tissue. In addition, studies using murine cystitis models have suggested that intracellular UPEC strains can persist within mouse bladders virtually undeterred in the face of antibiotic treatments that effectively reduce bacterial titers within the urine (Mulvey et al., 1998, 2000; Hvidberg et al., 2000). Recently, we have found that UPEC strains can persist at steady-state levels for multiple days within host bladder epithelial cells, but the bacteria also have the capacity to proliferate to high levels intracellularly (M.A.Mulvey, J.D.Schilling and S.J.Hultgren, in preparation). These data argue that the ability of UPEC to invade bladder epithelial cells has a critical role in the pathogenesis of UTIs. A mechanism by which UPEC can invade bladder epithelial cells has not been described. Scanning EM (SEM) studies of infected mouse bladders have revealed that type 1-piliated E.coli strains, but not isogenic mutants lacking the FimH adhesin, are able to induce localized changes in the host cell surface at points of contact with murine bladder epithelial cells (Mulvey et al., 1998). In addition, high-resolution EM has suggested that mouse bladder epithelial cells can envelop adherent bacteria, seemingly through contacts with the adhesive, FimH-containing tips of type 1 pili. These observations suggested that type 1 pilus-mediated attachment might have a role in the invasion of bladder cells by UPEC. Various studies have demonstrated that bacterial attachment can lead to the activation of host signal transduction cascades, sometimes inducing dramatic rearrangements of the eukaryotic cytoskeleton that can result in the internalization of adherent bacteria (Finlay and Falkow, 1997). Following attachment, some invasive bacteria can introduce effector molecules into their target host cells, triggering intense ruffling of the host cell membrane that results in bacterial uptake (Finlay and Cossart, 1997). In other cases, the host cell membrane can zipper around and envelope adherent bacteria in response to direct, sequential interactions between host cell membrane receptors and specific bacterial adhesins, which are sometimes referred to as invasins. The invasin protein encoded by Yersinia pseudotuberculosis (Isberg et al., 1987) and internalin A expressed by Listeria monocytogenes (Mengaud et al., 1996) are two prototypic invasins. Here, we present data indicating that the type 1 pilus adhesin FimH can function as an invasin, directly mediating bacterial invasion of bladder epithelial cells by inducing localized rearrangements of the host cell cytoskeleton. We also show that FimH-mediated bacterial invasion requires the activation of distinct host cell signaling events. Results Type 1 pili mediate invasion into bladder epithelial cells The ability of type 1-piliated E.coli strains to invade cultured human bladder epithelial cells (5637 cells) was examined by EM techniques. SEM of 5637 cells fixed 1 h after infection with a type 1-piliated clinical cystitis isolate, NU14, revealed that the host cell membrane could zipper around attached bacteria (Figure 1A). Like NU14, the recombinant laboratory K-12 strain AAEC185/pSH2 (type 1+) also adhered to 5637 cells and could be enveloped similarly by the host cell membrane (data not shown). The envelopment of NU14 and AAEC185/pSH2 by 5637 cells in vitro was morphologically similar to the envelopment of type 1-piliated E.coli by murine bladder cells in in vivo experiments (Mulvey et al., 1998). The uptake of NU14 and AAEC185/pSH2 by 5637 cells was confirmed by TEM studies (Figure 1B and C). Internalized bacteria were found predominantly within membrane-bound vacuoles. In contrast to NU14 and AAEC185/pSH2, the fimH− isogenic mutants, NU14-1 and AAEC185/pUT2002 (type 1+, FimH−) did not bind to, and were not enveloped by, 5637 cells as determined by EM and adherence assays (Figure 2C; data not shown). Figure 1.Invasion of bladder epithelial cells by type 1-piliated E.coli. 5637 bladder cells were examined by (A) SEM or (B and C) TEM 1–2 h after infection with NU14. Adherent bacteria were often observed being enveloped by bladder epithelial cells by SEM and could be detected within membrane-bound vacuoles by TEM. The scale bars indicate 1 μm. Download figure Download PowerPoint Figure 2.Type 1 pili mediate bacterial invasion of bladder cells. (A) Gentamicin protection assays indicate that NU14 can invade 5637 cells, while the isogenic fimH− mutant, NU14-1, was non-invasive. Addition of 2.5% D-mannose to the cell culture medium inhibited NU14 invasion. The plasmid pHJ20 (encoding FimH), but not pHJ19 (encoding FimH in the incorrect orientation), complements the invasion defect of NU14-1. (B) The non-invasive K-12 strain AAEC185 (type 1−), when transformed with the plasmid pSH2 (type 1+, FimH+), could also invade 5637 cells. AAEC185 strains transformed with pUT2002 (type 1+, FimH−) or pPIL110-35 (P pili+, with or without the PapG adhesin) were non-invasive. (C) Adherence assays indicate that AAEC185 expressing either wild-type type 1 pili (fimH+) or wild-type P pili (papG+) are able to adhere well to 5637 cells (46.2 ± 5.9% versus 16.5 ± 0.6%, respectively) in comparison with strains expressing type 1 or P pili lacking the FimH and PapG adhesins, respectively. (D) Calculated invasion indices (c.f.u. invaded/c.f.u. adherent) demonstrate that AAEC185 type 1 pili (FimH+) are ∼98% more efficient at mediating bacterial invasion than bacteria expressing P pili (PapG+). All data are representative of three or more independent assays, each performed in triplicate. Between experiments, the invasion frequencies of the type 1-piliated E.coli strains varied from 0.5 to 10%. This variability appears to be related to the passage number and status of the host cells and to the degree of bacterial piliation on the day of the experiment. Download figure Download PowerPoint The role of type 1 pili, and specifically the FimH adhesin, in mediating bacterial internalization by 5637 cells was investigated further using standard gentamicin protection assays (Elsinghorst, 1994; see Materials and methods). NU14 was found to invade 5637 cells via a mannose-inhibitable mechanism, while the isogenic fimH-negative mutant of NU14, NU14-1, was non-invasive (Figure 2A). Expression of fimH in trans within NU14-1 from plasmid pHJ20 restored the invasive phenotype. In contrast, the plasmid pHJ19, having fimH cloned in the incorrect orientation, was unable to complement the invasion defect of NU14-1. Several other type 1-piliated clinical E.coli cystitis isolates and the recombinant K-12 strain AAEC185/pSH2 (type 1+) were also tested for their ability to invade 5637 bladder cells. Like NU14, all type 1-piliated E.coli strains tested were able to invade 5637 cells (Figure 2B and data not shown). In contrast, the fimH-negative mutant AAEC185/pUT2002 (type 1+, fimH−) was non-invasive, as was the parent strain AAEC185, which has a complete deletion in the type 1 pilus gene cluster (Figure 2B and data not shown) (Blomfield, 1991). Invasion of 5637 cells by NU14 and other type 1-piliated strains occurred at levels that are comparable to the internalization frequencies of established invasive pathogens such as Listeria and Shigella (Braun et al., 1998; Mecsas et al., 1998). NU14 and AAEC185/pSH2 were also able to invade effectively other cultured human bladder epithelial cells, including T24, RT4 and J82 cells (data not shown). To test whether or not the observed internalization of type 1-piliated bacteria was the result of simply adhering to the host cells, we examined the ability of E.coli expressing P pili to adhere to and invade 5637 cells. P pili are encoded by the Pap (pyelonephritis-associated pili) gene cluster and mediate bacterial adhesion to Galα(1–4)Gal-containing host receptors through the P pilus-associated adhesin PapG (Leffler and Svanborg-Eden, 1980). AAEC185 was transformed with pPIL110-35 plasmids encoding the Pap gene cluster with or without papG (Stromberg et al., 1990). As shown in Figure 2B, AAEC185/pPIL110-35 (PapG+) was non-invasive even though the strain is able to adhere well to 5637 cells (Figure 2C). The papG− isogenic mutant was non-adherent and non-invasive. Comparison of the invasion indices (no. of c.f.u. invaded/no. of c.f.u. adhered) for type 1-piliated versus P-piliated AAEC185 indicated that adherence to 5637 cells in the absence of the FimH adhesin is not sufficient to trigger bacterial internalization into bladder epithelial cells (Figure 2D). FimH is sufficient to mediate bacterial internalization The ability of FimH to mediate directly internalization into bladder epithelial cells was tested using adhesin-coated polystyrene latex beads. Similar amounts of purified FimC–FimH chaperone–adhesin complexes, FimC alone or bovine serum albumin (BSA) were covalently attached to latex beads (∼1.17 μm in diameter). FimH, when bound to the FimC chaperone, can be purified easily in a native state that is able to bind mannose-containing host receptors (Choudhury et al., 1999). The ability of the protein-coated beads to be internalized by 5637 cells was examined by SEM and TEM. Following a 1 h incubation with FimCH-coated beads, SEM revealed numerous beads just beneath the bladder cell surface and in the process of internalization (Figure 3A and B). TEM indicated that the bladder cell membrane could ‘zipper’ around and eventually internalize attached FimCH-coated beads (Figure 3D–G). This zippering process appeared to be morphologically identical to that leading to the internalization of type 1-piliated E.coli (see Figure 1A; Mulvey et al., 1998). In contrast, BSA-coated beads and FimC-coated beads rarely adhered to the 5637 bladder cells and did not induce any apparent changes in the host cell membrane (Figure 3C and data not shown). Figure 3.Internalization of FimCH-coated beads. 5637 cells were incubated with FimCH- or BSA-coated beads for 30–60 min and then processed for SEM (A–C) or TEM (D–G). (A and B) FimCH-coated beads were internalized efficiently by 5637 cells, while the few adherent BSA-coated beads (C) did not induce any appreciable alterations in the 5637 cell membrane. (D–G) The host cell membrane appears to zipper around and eventually envelope FimCH-coated beads during the internalization process. Scale bars represent 5.0 μm (A and E), 0.5 μm (B–D) and 1.0 μm (F and G). Download figure Download PowerPoint The ability of FimCH-coated beads to be internalized by 5637 cells was quantified using an immunofluorescence-based assay (see Materials and methods). As shown in Figure 4, significant numbers of adherent FimCH-coated beads were internalized into 5637 cells within 60 min after addition to the cell culture wells. In contrast, the few BSA- and FimC-coated beads that occasionally adhered to 5637 cells were rarely internalized (Figure 4A). Like type 1-piliated bacteria, internalization of the FimCH-coated beads was inhibited by soluble D-mannose. However, D-mannose had no effect on the number of BSA- or FimC-coated beads that were internalized (Figure 4A). These results indicate that the type 1 pilus adhesin, FimH, cannot only mediate adherence to host cells, but is also sufficient to trigger internalization into human bladder cells in the absence of other bacterial factors. Figure 4.FimH is necessary and seemingly sufficient to mediate internalization. (A) 5637 cells, grown on sterile coverslips, were incubated with equal amounts of FimCH-, FimC- or BSA-coated beads ±2.5% D-mannose for 30–60 min. FimCH-coated beads were internalized in a D-mannose-inhibitable manner. In contrast, few control FimC- or BSA-coated beads were internalized and their uptake was not inhibited by D-mannose. Numbers of intracellular beads were determined as described in Materials and methods. Data are presented as an invasion index (total number of internalized beads divided by the total number of host cell-associated beads) and are representative of at least four independent assays. (B and C) Representative images of the internalization assay demonstrating the internalization of FimCH-coated beads into 5637 cells. (C) Fluorescent images (TRITC filter setting) were superimposed onto the corresponding phase contrast images such that extracellular beads appear red (arrow). The scale bar represents 5 μm. Download figure Download PowerPoint FimH-mediated invasion is dependent upon host actin polymerization The envelopment of type 1-piliated bacteria and FimCH-coated beads by 5637 cells appeared to involve substantial host cytoskeletal alterations, probably requiring actin rearrangements at sites of FimH-mediated contact with host cells. The role of actin in the invasion process was investigated using a specific inhibitor of F-actin polymerization, cytochalasin D. 5637 bladder cells were incubated with increasing concentrations of cytochalasin D for 30 min prior to infection with either NU14 or AAEC185/pSH2 (type 1+, fimH+). Cytochalasin D dramatically reduced FimH-mediated invasion (>99% inhibition; Figure 5A) but had no effect on bacterial adherence to the bladder cells (data not shown). Removal of the drug prior to infection made the host cells susceptible once again to type 1 pilus-mediated bacterial invasion, indicating that the effects of cytochalasin D were reversible (Figure 5A). The treatment of 5637 cells with cytochalasin D also inhibited the internalization of FimCH-coated beads (∼90% reduction; Figure 5B). Figure 5.Host cell actin polymerization plays an important role in the invasion process. (A) 5637 cells were pre-treated with cytochalasin D for 30 min prior to infection with AAEC185/pSH2. Gentamicin protection assays demonstrate that cytochalasin D (1 μg/ml) can completely abolish invasion. The effects of cytochalasin D are reversible if the host cells are washed with PBS prior to infection in fresh medium without drug. Similar results were obtained using NU14. (B) Cytochalasin D also inhibits the internalization of FimCH-coated beads. Download figure Download PowerPoint The effects of FimH-mediated adherence on the host cell actin cytoskeletal network were visualized using 5637 cells that were infected with green fluorescent protein (GFP)-expressing AAEC185/pSH2 or NU14. Following infection, 5637 cells were stained with Texas red-labeled phalloidin, a toxin that specifically binds to host F-actin. Confocal microscopy revealed that type 1-piliated bacteria (green) induced localized rearrangement of actin (red) at points of bacterial attachment (arrows, Figure 6B). Such localized alterations of the actin cytoskeleton were not observed in uninfected cells (Figure 6A). Like type 1-piliated E.coli, FimCH-coated beads, but not control BSA-coated beads, also induced localized changes of the actin cytoskeleton (data not shown). No large-scale, global changes, such as the formation of large actin stress fibers, were induced by FimH-mediated bacterial or bead attachment. In addition, type 1-piliated bacteria lacking the FimH adhesin did not bind 5637 cells and failed to induce any cytoskeletal changes (data not shown). These data suggest that FimH-mediated attachment is sufficient to induce localized actin polymerization. Figure 6.Actin rearrangements leading to internalization of type 1-piliated E.coli. (A) Texas red–phalloidin staining of uninfected 5637 cells highlighting cortical actin (red). (B) Localized actin polymerization is induced at points of contact (arrows) with AAEC185/pSH2/pcomGFP (type 1+, GFP+). (C and D) Images from (B) (arrows) and (E) are enlarged to show detail. The scale bar represents 2 μm. Download figure Download PowerPoint Invasion requires host protein tyrosine phosphorylation and PI 3-kinase activation Modification of the host actin cytoskeletal network can be influenced directly by the induction of specific host signaling events, including protein tyrosine phosphorylation and the activation of phosphoinositide 3-kinase (PI 3-kinase) (Guinebault et al., 1995; Hartwig et al., 1995; Carpenter and Cantley, 1996; Parsons, 1996). The involvement of host protein tyrosine phosphorylation in FimH-mediated bacterial invasion was tested using a potent general tyrosine kinase inhibitor, genistein, and a specific inhibitor of Src-family tyrosine kinases, PP1 (Gerwien et al., 1999). Treatment with genistein greatly inhibited the ability of both NU14 (Figure 7A) and AAEC185/pSH2 (data not shown) to invade 5637 bladder cells in a concentration-dependent fashion. In contrast, the inhibition of Src-family tyrosine kinases using PP1 had no effect on the ability of type 1-piliated E.coli to bind to and subsequently invade 5637 cells (Figure 7B). These data indicate that tyrosine kinases, other than members of the Src-family of kinases, are involved in FimH-mediated bacterial invasion. Figure 7.Inhibition of host protein tyrosine phosphorylation and PI 3-kinase activation diminishes invasion. (A) Gentamicin protection assays show that genistein prevents FimH-mediated bacterial internalization into 5637 cells in a concentration-dependent manner (>90% inhibition at 250 μM). (B) PP1, a Src-family tyrosine kinase inhibitor, had no effect on the internalization process. 5637 cells were treated with PP1 at concentrations that are known to inhibit Src-family kinase activity effectively (Gerwein et al., 1999). (C and D) Wortmannin and LY294002, two compounds that inhibit PI 3-kinase activity, can block FimH-mediated bacterial invasion of 5637 cells (∼90% inhibition at 100 nM and 50 μM, respectively). (E) Internalization of FimCH-coated beads into 5637 cells is also inhibited by wortmannin (∼80% inhibition at 100 nM). Download figure Download PowerPoint The role of PI 3-kinase in FimH-mediated bacterial invasion was investigated using two structurally unrelated compounds, wortmannin and LY294002, both of which inhibit PI 3-kinase activity (Vlahos et al., 1994). Treatment of 5637 cells with either 100 nM wortmannin or 50 μM LY294002 reduced FimH-mediated invasion of 5637 cells by ∼90% when compared with the control cells treated with solvent (dimethylsulfoxide; DMSO) alone (Figure 7C and D). Wortmannin also greatly inhibited the internalization of FimCH-coated beads by 5637 cells (Figure 7E). Neither wortmannin nor LY294002 had any effect on FimH-mediated bacterial adherence (data not shown). Induced formation of signaling and cytoskeletal complexes Previous work has shown that the p85α subunit of PI 3-kinase can interact with activated protein tyrosine kinases (Carpenter et al., 1990), including focal adhesin kinase (FAK) (Chen and Guan, 1994; Parsons, 1996). These interactions can lead eventually to local changes in the host actin cytoskeleton. Interactions between PI 3-kinase and FAK are facilitated by the phosphorylation of FAK at Tyr397 (Chen and Guan, 1996; Chen et al., 1996). Kinetic studies using a phospho-specific polyclonal anti-FAK antibody demonstrated that FAK becomes phosphorylated at Y397 within 5637 bladder cells shortly after infection with AAEC185/pSH2 (type 1+) (Figure 8A). Phosphorylation of FAK at Y397 correlated with the transient formation of complexes between FAK and PI 3-kinase, seen within 15 min after infection with AAEC185/pSH2 (Figure 8B). Induced phosphorylation of FAK at Y397 and the formation of complexes between FAK and PI 3-kinase were not detected in host cells after infection with the FimH− mutant AAEC185/pUT2002 (Figure 8A and B). Inhibitors that block FimH-mediated bacterial invasion (cytochalasin D, genistein, wortmannin and LY294002; Figure 7) were also found to inhibit the formation of complexes between FAK and PI 3-kinase (Figure 8C). The Src-family kinase inhibitor, PP1, which had no effect on FimH-mediated bacterial invasion, also had no effect on complex formation. These results indicate that phosphorylation of FAK at Y397, and the subsequent formation of complexes between FAK and PI 3-kinase, may be critical events in the signal transduction cascades that result in localized host actin cytoskeletal rearrangements and internalization of type 1-piliated E.coli. Figure 8.Type 1-piliated E.coli induce complex formation between FAK and PI 3-kinase and between vinculin and α-actinin. (A) Shortly after infection of 5637 cells with AAEC185/pSH2 (FimH+), blots probed with a phospho-specific anti-FAK antibody show that FAK becomes phosphorylated at Y397. Infection with AAEC185/pUT2002 (FimH−) did not induce phosphorylation of FAK Y397. (B) PI 3-kinase (the p85α subunit) co-precipitates with FAK within 15 min after infection with AAEC185/pSH2, but not after infection with AAEC185/pUT2002. (D) α-actinin, but not tensin or talin, transiently co-precipitates with vinculin beginning within 15 min after infection with AAEC185/pSH2, but not after infection with AAEC185/pUT2002. Control lysates containing tensin, talin and α-actinin were used to ensure that the different antibodies were functional in these assays. (C and E) Inhibitors of type 1 pilus-mediated bacterial invasion block complex formation between FAK and PI 3-kinase and between vinculin and α-actinin. The Src-family kinase inhibitor, PP1, had no effect on complex formation. Download figure Download PowerPoint PI 3-kinase and FAK can potentially modulate, directly or indirectly, a number of other molecules, including vinculin, tensin, talin and α-actinin, which can function in the reorganization and stabilization of the host actin cytoskeletal network (Parsons, 1996; Dekker and Segal, 2000). Earlier work has shown that α-actinin can function as a link between cross-linked actin filaments (Kuhlman et al., 1992) and vinculin (Kroemker et al., 1994), and it has been proposed that these interactions may serve to stabilize the cytoskeletal network. Co-immunoprecipitation experiments demonstrated that infection of 5637 cells with AAEC185/pSH2, but not with AAEC185/pUT2002, could stimulate the formation of transient complexes between vinculin and α-actinin (Figure 8D). However, infection with AAEC185/pSH2 did not appear to induce complex formation between α-actinin and either tensin or talin. Compounds that inhibit FimH-mediated bacterial invasion and the formation of complexes between FAK and PI 3-kinase (Figures 7 and 8C) also inhibited complex formation between vinculin and α-actinin, while PP1 had no effect (Figure 8E). These correlative data indicate that the formation of transient complexes between vinculin and α-actinin, like the formation of FAK–PI 3-kinase complexes, may be
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