The cell surface hyaluronate binding sites of invasive human bladder carcinoma cells
1987; Elsevier BV; Volume: 149; Issue: 1 Linguagem: Inglês
10.1016/0006-291x(87)91632-9
ISSN1090-2104
AutoresRaymond E. Nemec, Bryan P. Toole, Warren Knudson,
Tópico(s)Fibroblast Growth Factor Research
ResumoHigh-affinity, cell surface binding sites for hyaluronate were demonstrated on highly invasive human bladder carcinoma cells. These binding sites were shown to be specific for hyaluronate, saturable and exhibit a Km of 0.94 × 10−9M and a Bmax of 65ng hyaluronate/ 106 cells. The binding of [3H]hyaluronate to a fixed cell-affinity column was competed with unlabeled hyaluronate and hyaluronate-hexasaccharide but not with hyaluronate-tetrasaccharide, chondroitin sulfate, heparin or non-sulfated dextran. Pre-treatment of cells with protease destroyed the binding activity whereas pre-treatment with Streptomyces hyaluronidase to reveal occupied binding sites had no effect. No hyaluronate-binding activity was observed on normal human fibroblasts.
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