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Three novel single nucleotide polymorphisms in UGT1A10

2002; Elsevier BV; Volume: 17; Issue: 5 Linguagem: Inglês

10.2133/dmpk.17.488

1880-0920

Mayumi Saeki, Shogo Ozawa, Yoshiro Saito, Hideto Jinno, Tetsuya Hamaguchi, Hiroshi Nokihara, Yasuhiro Shimada, Hideo Kunitoh, Noboru Yamamoto, Yuichiro Ohe, Yasuhide Yamada, Kuniaki Shirao, Manabu Muto, Kiyomi Mera, Kōichi Goto, Hironobu Ohmatsu, Kaoru Kubota, Seiji Niho, Ryutaro Kakinuma, Hironobu Minami, Atsushi Ohtsu, Teruhiko Yoshida, Nagahiro Saijo, Jun‐ichi Sawada,

Synthesis and Biological Evaluation

Three novel single nucleotide polymorphisms (SNPs) were found in the UDP-glucuronosyltransferase (UGT) 1A10 gene from 24 Japanese patients with various cancers who were administered the anti-tumor drug, irinotecan (CPT-11). The detected SNPs were as follows: 1) SNP, MPJ6_U1A003; GENE NAME, UGT1A10; ACCESSION NUMBER, AF297093; LENGTH, 25 bases; 5'-CAGATGCCATGAC/TTTTCAAGGAGAG-3'. 2) SNP, MPJ6_U1A004; GENE NAME, UGT1A10; ACCESSION NUMBER, AF297093; LENGTH, 25 bases; 5'-CCTAGAAATAGCC/TTCTGAAATTCTC-3'. 3) SNP, MPJ6_U1A030; GENE NAME, UGT1A10; ACCESSION NUMBER, AF297093; LENGTH, 25 bases; 5'-GGTTGTAGTCATG/ACCAGAGGTGAGT-3' All the three SNPs were located in exon 1 and their frequencies were all 0.021. Among these SNPs, MPJ6_U1A003 and U1A030 resulted in amino acid alterations, T202I and M59I, respectively. The third SNP, MPJ6_U1A004, introduced a synonymous amino acid change (A231A).