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Response of Rat Type II Pneumocyte Na,K-ATPase to Hyperoxic Injury

1991; Elsevier BV; Volume: 99; Issue: 3 Linguagem: Inglês

10.1378/chest.99.3_supplement.31s

1931-3543

Linda Nici, R. Dowin, Maureen Gilmore-Hebert, James D. Jamieson, David H. Ingbar,

Neonatal Respiratory Health Research

A major function of the alveolar epithelium is to keep the air space free of fluid and preserve gas exchange. Na, K-ATPase has been implicated as an important mechanism in sodium and fluid absorption from the alveolar space. 1 Staub NC Pulmonary edema. Physiol Rev. 1974; 54: 678-811 Crossref PubMed Google Scholar , 2 Crandall ED Staub NC Goldberg MD Effros RM Recent developments in pulmonary edema. Ann Intern Med. 1983; 99: 808-822 Crossref PubMed Scopus (38) Google Scholar , 3 Staub NC Alveolar flooding and clearance. Am Rev Respir Dis. 1983; 127: 544-551 Google Scholar , 4 Matthay MA Lendolt CC Staub NC Differential liquid and protein clearance from the alveoli of anesthetized sheep. J Appl Physiol. 1982; 53: 96-104 Crossref PubMed Scopus (174) Google Scholar , 5 Crandall ED Palumbo RL Goodman BE Effects of terbutaline on sodium flux from alveolar to vascular fluid in isolated perfused rat lung. Am Rev Respir Dis. 1984; 129: 345 Google Scholar , 6 Crandall ED Fluid balance across alveolar epithelium. Am Rev Respir Dis. 1983; 127: S2-S59 Crossref Scopus (23) Google Scholar , 7 Mason RJ Williams MC Widdicombe JH Sanders MJ Misfeld DS Berry LC Transepithelial transport by pulmonary alveolar type II cells in primary culture. Proc Natl Acad Sci USA. 1982; 79: 6033-6037 Crossref PubMed Scopus (163) Google Scholar , 8 Goodman BE Fleischer RS Crandall ED Evidence for active Na+ transport by cultured monolayers of pulmonary alveolar epithelial cells. Am J Physiol. 1983; 245 (Cell Physiol 14): C78-C83 PubMed Google Scholar In the rat lung it has been localized to the basolateral surface of type II cells, which suggests that these cells play a major role in keeping the alveolus free of fluid. 9 Schneeberger EE McCartney KM Cytochemical localization of Na, K-ATPase in rat type II pneumocytes. J Appl Physiol. 1986; 60: 1584-1589 Crossref PubMed Scopus (10) Google Scholar In normal lung development as well as renal pathologic states, sodium pump expression and activity increase with increased demands for fluid and sodium resorption. 10 Orlowski T Lingrel JB Tissue-specific and developmental regulation of rat Na, K-ATPase catalytic alpha isoform and beta subunit mRNAs. J Biol Chem. 1988; 263: 10436-10442 PubMed Google Scholar , 11 Obando MA Marin R Proverbio T Proverbio F High sodium diet and Na+-stimulated ATPase activities in basolateral plasma membranes from rat kidney proximal tubular cells. Biochem Pharmacol. 1987; 36: 7-11 Crossref PubMed Scopus (7) Google Scholar , 12 Scherzer H Wald H Czaczkes JW Na, K-ATPase in isolated rabbit tubules after unilateral nephrectomy and Na+ loading. Am J Physiol. 1985; 248: F565-F573 PubMed Google Scholar The alveolar epithelium may respond in a similar fashion to pulmonary edema. We examined the role of Na, K-ATPase in hyperoxic lung injury during the phases of injury and repair. A variety of techniques were employed to examine this question, including wet to dry lung weights, light and electron microscopy, immunofluorescence and immuno-electron microscopy, and Northern and Western blot analysis.