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Dog saliva – an important source of dog allergens

2013; Wiley; Volume: 68; Issue: 5 Linguagem: Inglês

10.1111/all.12130

1398-9995

Natalija Polović, Konrad Wadén, Jonas Binnmyr, Carl Hamsten, R. Grönneberg, Carina Palmberg, Natalija Milčić-Matić, Tomas Bergman, Hans Grönlund, Marianne van Hage,

Food Allergy and Anaphylaxis Research

Abstract Background Allergy to dog ( C anis familiaris ) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of I g E ‐mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE‐binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry ( LC ‐ MS / MS ) using pooled or individual sera from dog‐allergic patients ( n = 13). Sera from 59 patients I g E positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for I g E against saliva and dander by ELISA . Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog‐allergic patients. Additionally, I g E ‐binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of I g E ‐binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, C an f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog dander–positive sera ( n = 44) were I g E positive to dog saliva. Among patients I g E negative to dander, but with symptoms to dog, 20% were I g E positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The I g E ‐binding protein profile of saliva from different dogs varies.