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Raman Spectroscopic Analysis Identifies Testicular Microlithiasis as Intratubular Hydroxyapatite

2004; Lippincott Williams & Wilkins; Volume: 171; Issue: 1 Linguagem: Inglês

10.1097/01.ju.0000101948.98175.94

1527-3792

Bas W.D. de Jong, Carlos A. de Gouveia Brazao, Hans Stoop, Katja P. Wolffenbuttel, J. Wolter Oosterhuis, G.J. Puppels, R. F. A. Weber, Leendert H. J. Looijenga, Dirk J. Kok,

Testicular diseases and treatments

No AccessJournal of UrologyCLINICAL UROLOGY: Original Articles1 Jan 2004Raman Spectroscopic Analysis Identifies Testicular Microlithiasis as Intratubular Hydroxyapatite B.W.D. de JONG, C.A. De GOUVEIA BRAZAO, H. STOOP, K.P. WOLFFENBUTTEL, J.W. OOSTERHUIS, G.J. PUPPELS, R.F.A. WEBER, L.H.J. LOOIJENGA, and D.J. KOK B.W.D. de JONGB.W.D. de JONG , C.A. De GOUVEIA BRAZAOC.A. De GOUVEIA BRAZAO , H. STOOPH. STOOP , K.P. WOLFFENBUTTELK.P. WOLFFENBUTTEL , J.W. OOSTERHUISJ.W. OOSTERHUIS , G.J. PUPPELSG.J. PUPPELS , R.F.A. WEBERR.F.A. WEBER , L.H.J. LOOIJENGAL.H.J. LOOIJENGA , and D.J. KOKD.J. KOK View All Author Informationhttps://doi.org/10.1097/01.ju.0000101948.98175.94AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: As diagnosed by ultrasonography, testicular microlithiasis is associated with various benign and malignant conditions. The molecular constitution of these microliths is largely unknown. Raman spectroscopy provides detailed in situ information about the molecular composition of tissues and to our knowledge it has not been applied to gonadal microliths. We analyzed the molecular composition of gonadal microlithiasis and its surrounding region using Raman spectroscopy in malignant and benign conditions. Materials and Methods: Multiple microliths from 6 independent samples diagnosed with gonadal microlithiasis by ultrasound and histologically confirmed were investigated by Raman spectroscopy. The samples included 4 testicular parenchyma samples adjacent to a germ cell tumor (4 seminomas), a gonadoblastoma of a dysgenetic gonad and testicular biopsy of a subfertile male without malignancy. Results: Raman spectroscopic mapping demonstrated that testicular microliths were located within the seminiferous tubule. Glycogen surrounded all microliths in the samples associated with germ cell neoplasm but not in the benign case. The molecular composition of the 26 microliths in all 6 conditions was pure hydroxyapatite. Conclusions: Microliths in the testis are located in the seminiferous tubules and composed of hydroxyapatite. In cases of germ cell neoplasm they co-localize with glycogen deposits. References 1 : Testicular microliths: their origin and structure. J Urol1980; 124: 105. Link, Google Scholar 2 : Testicular microlithiasis in 2 children with bilateral cryptorchidism. J Urol1979; 121: 535. Link, Google Scholar 3 : Sonographic appearance of testicular microlithiasis. Eur Urol1993; 24: 251. Google Scholar 4 : Testicular microlithiasis: imaging appearances and pathologic correlation. Radiology1994; 192: 781. Google Scholar 5 : Testicular microlithiasis: a benign condition with a malignant association. J Clin Ultrasound1996; 24: 197. 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Google Scholar From the Departments of Paediatric Urology (BWDDJ, KPW, DJK), Andrology (CADGB, RFAW), Urology (CADGB, RFAW), Pathology/Laboratory for Experimental Patho-Oncology/Josephine Nefkens Institute (CADGB, HS, JWO, LHJL) and General Surgery (GJP), Erasmus Medical Center, University Medical Center, Rotterdam, The Netherlands© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 171Issue 1January 2004Page: 92-96 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Keywordslithiasisdurapatitespectrum analysis, RamantestisMetricsAuthor Information B.W.D. de JONG More articles by this author C.A. De GOUVEIA BRAZAO More articles by this author H. STOOP More articles by this author K.P. WOLFFENBUTTEL More articles by this author J.W. OOSTERHUIS More articles by this author G.J. PUPPELS More articles by this author R.F.A. WEBER More articles by this author L.H.J. LOOIJENGA More articles by this author D.J. 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