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Immunohistochemical staining for P1 and P2 promoter-driven hepatocyte nuclear factor-4α may complement mucin phenotype of differentiated-type early gastric carcinoma

2009; Wiley; Volume: 59; Issue: 7 Linguagem: Inglês

10.1111/j.1440-1827.2009.02394.x

1440-1827

Kabuto Takano, Go Hasegawa, Shuying Jiang, Isao Kurosaki, Katsuyoshi Hatakeyama, Hiroko Iwanari, Toshiya Tanaka, Takao Hamakubo, Tatsuhiko Kodama, Makoto Naito,

Genetic factors in colorectal cancer

Pathology InternationalVolume 59, Issue 7 p. 462-470 Immunohistochemical staining for P1 and P2 promoter-driven hepatocyte nuclear factor-4α may complement mucin phenotype of differentiated-type early gastric carcinoma Kabuto Takano, Kabuto Takano Divisions of Cellular and Molecular Pathology and Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorGo Hasegawa, Go Hasegawa Divisions of Cellular and Molecular Pathology andSearch for more papers by this authorShuying Jiang, Shuying Jiang Divisions of Cellular and Molecular Pathology and Perseus Proteomics andSearch for more papers by this authorIsao Kurosaki, Isao Kurosaki Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorKatsuyoshi Hatakeyama, Katsuyoshi Hatakeyama Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorHiroko Iwanari, Hiroko Iwanari Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorToshiya Tanaka, Toshiya Tanaka Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorTakao Hamakubo, Takao Hamakubo Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorTatsuhiko Kodama, Tatsuhiko Kodama Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorMakoto Naito, Corresponding Author Makoto Naito Divisions of Cellular and Molecular Pathology andMakoto Naito, MD, PhD, Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Sciences, Asahimachidori 1-757, Chuou-ku, Niigata 951-8510, Japan. Email: [email protected]Search for more papers by this author Kabuto Takano, Kabuto Takano Divisions of Cellular and Molecular Pathology and Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorGo Hasegawa, Go Hasegawa Divisions of Cellular and Molecular Pathology andSearch for more papers by this authorShuying Jiang, Shuying Jiang Divisions of Cellular and Molecular Pathology and Perseus Proteomics andSearch for more papers by this authorIsao Kurosaki, Isao Kurosaki Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorKatsuyoshi Hatakeyama, Katsuyoshi Hatakeyama Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata,Search for more papers by this authorHiroko Iwanari, Hiroko Iwanari Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorToshiya Tanaka, Toshiya Tanaka Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorTakao Hamakubo, Takao Hamakubo Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorTatsuhiko Kodama, Tatsuhiko Kodama Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, JapanSearch for more papers by this authorMakoto Naito, Corresponding Author Makoto Naito Divisions of Cellular and Molecular Pathology andMakoto Naito, MD, PhD, Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Sciences, Asahimachidori 1-757, Chuou-ku, Niigata 951-8510, Japan. Email: [email protected]Search for more papers by this author First published: 22 June 2009 https://doi.org/10.1111/j.1440-1827.2009.02394.xCitations: 14Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Hepatocyte nuclear factor 4α (HNF4α) isoforms in the human stomach have not been fully investigated. The purpose of the present study was to evaluate the expression of P1 and P2 promoter-driven HNF4α (P1 and P2-HNF4α) in differentiated-type early gastric carcinomas (DEGC). P1- and P2-HNF4α expression was examined immunohistochemically both in non-neoplastic mucosa and carcinoma from surgical specimens. In all samples of non-neoplastic mucosa, foveolar, cardiac, fundic and pyloric gland epithelium was negative for P1-HNF4α, but was positive for P2-HNF4α. Intestinal metaplasia was positive for P1 and P2-HNF4α in all cases. Gastric carcinomas were classified into four mucin phenotypes based on the pattern of mucin expression: gastric, intestinal, mixed and null type. DEGC showed striking differences in the staining pattern for P1-HNF4α according to the mucin phenotype. Gastric carcinomas of intestinal, mixed and null type showed high positivity for P1-HNF4α, but the gastric type was negative for P1-HNF4α in all but one tumor. In contrast, P2-HNF4α was expressed in all tumors regardless of the mucin phenotype. 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