INFLUENCE OF FREEZE-STORAGE ON NUCLEIC ACID CONCENTRATIONS IN BACTERIA AND DUODENAL DIGESTA
1984; Canadian Science Publishing; Volume: 64; Issue: 3 Linguagem: Inglês
10.4141/cjas84-090
ISSN1918-1825
Autores Tópico(s)Digestive system and related health
ResumoSeparation of bacteria from duodenal digesta prior to storage (−25 °C) resulted in 17 and 27% loss of RNA at 1 and 2 weeks, respectively. Storage of total digesta, with bacterial separation immediately prior to analysis, prevented loss of RNA. Purine base concentrations were not influenced by storage. However, removal of free purine base in digesta prior to analysis is recommended. Key words: Nucleic acids, freeze-storage, bacteria, duodenal digesta
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