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Peptide maps of antibodies against an antigen containing two different determinant groups

1965; Elsevier BV; Volume: 18; Issue: 1 Linguagem: Inglês

10.1016/0006-291x(65)90885-5

1090-2104

Eugene F. Gold, Katherine L. Knight, Felix Haurowitz,

Cell Adhesion Molecules Research

Abstract Bovine serum albumin doubly labelled by coupling with equivalent amounts of diazotized arsanilic acid (Ars) and p-aminophenyl-N-trimethyl-ammonium chloride (R 4 N) was injected into rabbits. Hapten-specific antibodies against the two determinants were separated from each other and from anti-BSA. Peptide maps of anti-Ars and anti-R 4 N differ from each other only in one or two spots. Large portions of the two types of antibody molecules seem to be identical. Since both haptens are bound to the same BSA molecules, we assume that they are initially incorporated into the same cells. It is not yet known whether anti-Ars and anti-R 4 N are formed in the same cells or whether the haptens, after fragmentation of the antigen, are transferred to different antibody forming cells.