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Cooperative Epigenetic Modulation by Cancer Amplicon Genes

2010; Cell Press; Volume: 18; Issue: 6 Linguagem: Inglês

10.1016/j.ccr.2010.11.013

1878-3686

Lixin Rui, N. C. Tolga Emre, Michael J. Kruhlak, Hye-Jung Chung, Christian Steidl, Graham W. Slack, George W. Wright, Georg Lenz, Vu N. Ngo, Arthur L. Shaffer, Weihong Xu, Hong Zhao, Yandan Yang, Laurence Lamy, R. Eric Davis, Wenming Xiao, John Powell, David J. Maloney, Craig J. Thomas, Peter Möller, Andreas Rosenwald, German Ott, Hans Konrad Müller‐Hermelink, Kerry J. Savage, Joseph M. Connors, Lisa M. Rimsza, Elı́as Campo, Elaine S. Jaffe, Jan Delabie, Erlend B. Smeland, Dennis D. Weisenburger, Wing C. Chan, Randy D. Gascoyne, David Levens, Louis M. Staudt,

Cancer-related gene regulation

Chromosome band 9p24 is frequently amplified in primary mediastinal B cell lymphoma (PMBL) and Hodgkin lymphoma (HL). To identify oncogenes in this amplicon, we screened an RNA interference library targeting amplicon genes and thereby identified JAK2 and the histone demethylase JMJD2C as essential genes in these lymphomas. Inhibition of JAK2 and JMJD2C cooperated in killing these lymphomas by decreasing tyrosine 41 phosphorylation and increasing lysine 9 trimethylation of histone H3, promoting heterochromatin formation. MYC, a major target of JAK2-mediated histone phosphorylation, was silenced after JAK2 and JMJD2C inhibition, with a corresponding increase in repressive chromatin. Hence, JAK2 and JMJD2C cooperatively remodel the PMBL and HL epigenome, offering a mechanistic rationale for the development of JAK2 and JMJD2C inhibitors in these diseases.