Crystallization of human β-hexosaminidase B
1992; Elsevier BV; Volume: 227; Issue: 2 Linguagem: Inglês
10.1016/0022-2836(92)90911-3
ISSN1089-8638
AutoresW. Bret Church, Lora Swenson, Michael N.G. James, Don J. Mahuran,
Tópico(s)Enzyme Production and Characterization
Resumoβ-Hexosaminidase is a lysosomal hydrolase that is important in the metabolism of sphingoglycolipids. β-Hexosaminidase B and β-hexosaminidase A are the major isozymes in normal human tissue. β-Hexosaminidase B is a homodimer of β subunits, and β-hexosaminidase A is a heterodimer composed of an α and a β subunit. Crystals of β-hexosaminidase B (Mr 112,000) have been grown using the hanging drop technique. They are elongated hexagonal prisms with maximum dimensions of 0.2 mm × 0.2 mm × 0.7 mm. The space group is P6122 (or enantiomorph): the unit cell dimensions are a = b = 114.2 Å, c = 402.2 Å, α = β = 90 °, γ = 120 °. The molecular mass and cell dimensions suggest that there is one dimer per asymmetric unit. Crystals diffract to 3.2 Å resolution.
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