Artigo Acesso aberto Produção Nacional Revisado por pares

Preliminary evaluation of Hitchens–Pike–Todd–Hewitt medium (HPTH) for detection of group B streptococci in pregnant women

2010; Wiley; Volume: 24; Issue: 6 Linguagem: Inglês

10.1002/jcla.20419

ISSN

1098-2825

Autores

Maurício Chaves, Rúbia Andreia Faleiros de Pádua, Paula A.Z. Campanerut, Sandra Marisa Pelloso, Maria Dalva de Barros Carvalho, Vera Lúcia Dias Siqueira, Regiane Bertin de Lima Scodro, Rosilene Fressatti Cardoso,

Tópico(s)

Sepsis Diagnosis and Treatment

Resumo

Abstract We aimed to compare Hitchens–Pike–Todd–Hewitt (HPTH) enrichment broth, Todd–Hewitt broth, and direct culture onto blood sheep agar containing selective antimicrobial (ABA) in screening Group B Streptococci (GBS) carriage in pregnant women. From April to June 2007, duplicate lower vaginal and duplicate anorectal specimens of 102 pregnant women at 35 weeks of gestation or more, who were assisted at Santa Casa de Misericórdia Hospital, Maringá, Paraná, Brazil, were screened by GBS using HPTH, Todd–Hewitt broth, and ABA. Twenty‐five(24.5%) pregnant women had one or more positive culture for GBS in those media. The positive rate for each medium was 21.6% (22/102) in the HPTH, 12.8% (13/102) in ABA, and 11.8% (12/102) in Todd–Hewitt broth. HPTH seems to be a high satisfactory medium for screening GBS in vaginal and anorectal samples in pregnant women and additional studies would be interesting in a larger number of pregnant women and in different laboratories. J. Clin. Lab. Anal. 24:403–406, 2010. © 2010 Wiley‐Liss, Inc.

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