Chromosomes bearing amplified genes are a preferential target of chemicals inducing chromosome breakage and aneuploidy
1993; Elsevier BV; Volume: 301; Issue: 3 Linguagem: Inglês
10.1016/0165-7992(93)90071-3
ISSN1878-7053
AutoresLaura Ottagio, Stefania Bonati, Zita Cavalieri, Angelo Abbondandolo,
Tópico(s)Carcinogens and Genotoxicity Assessment
ResumoMicronuclei were induced in V79 Chinese hamster cells and in PALA L and MTX M, two derivative cell lines harboring amplified genes, with 1,3-bis(2-chloroethyl)nitrosourea (BCNU) and vinblastine. Spontaneous and induced micronuclei were analyzed for the presence of centromeres by immunofluorescent CREST staining. Micronuclei formed in PALA L cells were also analyzed for the presence of amplified DNA by in situ hybridization with a CAD gene probe. Both cell lines containing amplified genes showed increased micronucleus induction by BCNU and vinblastine. The marker chromosome of PALA L cells was found to be a preferential target for both the clastogenic and the aneugenic action of the two chemicals, DNA amplification seems therefore to be a destabilizing factor of chromosomal structural integrity and mitotic segregation.
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