Different requirements for the induction of antibody-dependent and immune complexes triggered cytotoxicity mediated by monocytes
1988; Elsevier BV; Volume: 17; Issue: 2 Linguagem: Inglês
10.1016/0165-2478(88)90077-6
ISSN1879-0542
AutoresMirta Giordano, Jorge Geffner, G Serebrinsky, Marina S. Palermo, Martin A. Isturiz,
Tópico(s)Pharmacogenetics and Drug Metabolism
ResumoWe have previously shown that immune complexes triggered nonspecific cytotoxicity (NSC) towards nonsensitized target cells and antibody-dependent cellular cytotoxicity (ADCC), two functions mediated through monocyte Fcγ receptors, employing different lytic mechanisms [Geffner, J. R., et al. (1986) Clin. Exp. Immunol. 67, 646]. In this report, we analyze some of the metabolic requirements involved in the induction of monocyte NSC and ADCC. The results showed NSC to be dependent on: (1) metabolic energy derived from glycolysis, (2) availability of external Ca2+, (3) calmodulin activity, (4) integrity of microtubules, but not the microfilament system, and (5) activation of phospholipase(s) and lipoxygenase. On the other hand, ADCC was not impaired by: (1) inhibition of glycolysis, (2) Ca2+ chelation, (3) disruption of microtubules, or (4) inhibition of calmodulin or lipoxygenase. It is concluded that monocyte NSC and ADCC are regulated by different endogenous signals.
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