Voltar
Artigo Revisado por pares
BIBTEX RIS

A new serotype of Bacillus thuringiensis isolated in Germany: Bacillus thuringiensis var. darmstadiensis

1968; Elsevier BV; Volume: 10; Issue: 2 Linguagem: Inglês

10.1016/0022-2011(68)90104-3

ISSN

1096-0805

Autores

Aloysius Krieg, H. de Barjac, A Bonnefoi,

Tópico(s)

Insect and Pesticide Research

Resumo

14C-labelled Bacillus thuringiensis var. darmstadiensis (Btd) crystals were used to detect crystal-associated protease activity. Optimal protease activity occurred at pH 8.4 and 9.7, and at 15 and 35°C. Protease activity was destroyed by heating the crystals at 121°C for 15 min. Cellular enzymes from Btd did not solubilise Btd crystals. Iodoacetic acid (50 mM) caused a 20% reduction in protease activity while phenylmethyl-sulphonylfluoride (PMSF), ethylenediamine-tetraacetic acid (EDTA) and p-chloromercuribenzene (PCMB) had no inhibitory effect on protease activity.

Referência(s)