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Intracellular Translocation of Iodine-125-Labeled Insulin: Direct Demonstration in Isolated Hepatocytes

1978; American Association for the Advancement of Science; Volume: 200; Issue: 4343 Linguagem: Inglês

10.1126/science.644321

1095-9203

Phillip Görden, J L Carpentier, P Freychet, A. LeCam, Lelio Orci,

Cellular transport and secretion

Insulin labeled with iodine-125 binds to receptors on isolated rat hepatocytes. At low temperatures initial binding is restricted to the plasma membrane as detected by direct quantitative autoradiographic analysis with the electron microscope. With increasing time and temperature of incubation there is a systematic and progressive translocation of autoradiographic grains to a highly limited area of the cell periphery representing no more than 15 percent of the radius of the cell.