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On-Chip High-Pressure Picoliter Injector for Pressure-Driven Flow through Porous Media

2004; American Chemical Society; Volume: 76; Issue: 17 Linguagem: Inglês

10.1021/ac0493572

1520-6882

David. Reichmuth, Timothy J. Shepodd, Brian J. Kirby,

Electrohydrodynamics and Fluid Dynamics

A high-pressure (>3 MPa) on-chip injector has been developed for microchip applications including HPLC. The mechanical injector is implemented using in situ photopolymerization of fluorinated acrylates inside wet-etched silica microchips. The injector allows reproducible injections as small as 180 pL with <250 ms duration. The injector operated robustly over 60 days and over 1000 injections. The injector is unique among polymer-based valves as it functions in aqueous, acetonitrile, and mixed buffers at high pressures without detectable leakage.