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Solid-phase extraction of isosorbide dinitrate and two of its metabolites from plasma for gas chromatographic analysis

1995; Elsevier BV; Volume: 665; Issue: 2 Linguagem: Inglês

10.1016/0378-4347(94)00543-e

1872-812X

I. Gremeau, Valérie Sautou, V. Pinon, F. Rivault, Joël Chopineau,

Antibiotics Pharmacokinetics and Efficacy

A rapid, accurate and selective method for the determination of isosorbide dinitrate and its 2- and 5-isosorbide mononitrate metabolites in 1.0 ml of human plasma has been developed. Before chromatographic quantitation by gas-liquid chromatography with electron-capture detection, the compounds are subjected to solid-phase extraction, using ENVI 18 cartridges (Supelco). The intra-day and inter-day coefficients of variation are less than 10%, except the inter-day coefficient of variation for the assay of 5-isosorbide dinitrate which is less than 15%. Limits of quantitation are 10, 10 and 20 ng/ml for isosorbide dinitrate, 2-isosorbide mononitrate and 5-isosorbide mononitrate, respectively. Recoveries are in excess of 90% for isosorbide dinitrate and 70% for its two metabolites.