Oligodendrocyte-type-2 astrocyte (O-2A) progenitors increase the survival of rat mesencephalic, dopaminergic neurons from death induced by serum deprivation
1994; Elsevier BV; Volume: 166; Issue: 2 Linguagem: Inglês
10.1016/0304-3940(94)90480-4
ISSN1872-7972
AutoresTakao Takeshima, Jane M. Johnston, John W. Commissiong,
Tópico(s)Neuroinflammation and Neurodegeneration Mechanisms
ResumoWhen a primary culture of E16 rat striatal cells was grown in a serum-free medium, treatment with basic fibroblast growth factor (bFGF, 10 ng/ml) caused the generation of the progenitor cell for oligodendrocytes and type-2 astrocytes (O-2A). Immunostaining tests confirmed that > 90% of the cells were positive for A2B5, and < 5% positive for glial fibrillary acidic protein (GFAP). When E14, mesencephalic, dopaminergic neurons were co-cultured with established O-2A progenitor cells in a serum-free growth medium, the survival of tyrosine hydroxylase-positive (TH+) neurons increased 23-fold and 668-fold at the 5th and 10th days, respectively, compared with control cultures plated on poly-d-lysine. Conditioned medium from the O-2A progenitor cultures also decreased the death of TH+ neurons. The mitotic inhibitor, cytosine arabinoside (1.0 μM), did not block the protective effect of the O-2A progenitor cells. O-2A progenitor cells produce a potent, soluble factor, that mediates the increased survival of dopaminergic neurons in vitro.
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