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Monoclonal antibodies against corn stunt spiroplasma

1985; Canadian Science Publishing; Volume: 31; Issue: 10 Linguagem: Inglês

10.1139/m85-169

1480-3275

Chan‐Pin Lin, T. A. Chen,

Toxin Mechanisms and Immunotoxins

Monoclonal antibodies against the corn stunt spiroplasma were produced by fusing NS-1/1-Ag4-1 murine myeloma cells with splenic cells from mice immunized with corn stunt spiroplasma (I-747). Isotypes of these monoclonal antibodies have been determined to be IgG1 or IgG2a subclass by the Ouchterlony double diffusion method using class and subclass specific antibodies for mouse IgG1, IgG2a, IgG2b, IgG3, and IgM. Antibody titers for both hybridoma culture supernatants and ascitic fluids were determined with indirect biotinylated enzyme-linked immunosorbent assay and they ranged from 800 to 3200, and 312 500 to 7 812 400, respectively. In serological specificity studies, with indirect enzyme-linked immunosorbent assay, 32 spiroplasma strains belonging to 19 different serogroups were tested using both corn stunt spiroplasma monoclonal antibodies and conventionally produced polyclonal antibodies. Corn stunt spiroplasma specific monoclonal antibodies reacted specifically with any of the three corn stunt spiroplasma strains tested, but not with all of the other spiroplasma strains from different groups and subgroups. On the other hand, some nonspecific reactions to heterologous spiroplasma strains were obtained when conventional polyclonal antibodies were used.