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Detection of Panulirus argus Virus 1 (PaV1) in exported frozen tails of subadult-adult Caribbean spiny lobsters Panulirus argus

2009; Inter-Research; Volume: 86; Linguagem: Inglês

10.3354/dao02117

1616-1580

Juan Pablo Huchín-Mian, Patricia Briones‐Fourzán, R Simá-Álvarez, Yanis Cruz‐Quintana, Juan Antonio Pérez-Vega, Enrique Lozano‐Álvarez, Cristina Pascual, Rossanna Rodríguez‐Canul,

Crustacean biology and ecology

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 86:159-162 (2009) - DOI: https://doi.org/10.3354/dao02117 NOTE Detection of Panulirus argus Virus 1 (PaV1) in exported frozen tails of subadult-adult Caribbean spiny lobsters Panulirus argus Juan Pablo Huchin-Mian1, Patricia Briones-Fourzán2, Raúl Simá-Álvarez1, Yanis Cruz-Quintana1, Juan Antonio Pérez-Vega1, Enrique Lozano-Álvarez2, Cristina Pascual-Jiménez2,3, Rossanna Rodríguez-Canul1,* 1Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad Mérida, Km 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán 97310, Mexico 2Universidad Nacional Autónoma de México, Instituto de Ciencias del Mar y Limnología, Unidad Académica Puerto Morelos, Ap Postal 1152, Cancún, Quintana Roo 77500, Mexico 3Universidad Nacional Autónoma de México, Unidad Marina de Ciencias Interdisciplinarias Unidad Sisal, Yucatán 97355, Mexico *Corresponding author. Email: rossana@mda.cinvestav.mx ABSTRACT: The Caribbean spiny lobster Panulirus argus is a valuable fishing resource and the trade in frozen lobster tails is an important industry. However, the presence of the pathogenic virus Panulirus argus Virus 1 (PaV1), which causes systemic infection in P. argus and is particularly lethal to juvenile individuals, has not been previously examined in imported/exported lobster products. We used PCR assays to determine the presence of PaV1 in abdominal muscle tissue of 22 frozen P. argus tails exported from Belize to Mexico. Based on their size, the tails belonged to subadult–adult lobsters. Using specific primers targeted for PaV1 resulted in 11 tails showing a specific 499 bp band. The sequence of positive amplified fragments showed a high similarity to PaV1 (95% identity with GenBank accession no. EF206313.1). Although the pathogenicity of PaV1 was not evaluated in the present study, our results provide the first evidence of PaV1 in frozen lobster tails exported in the seafood industry as well as the first molecular evidence of PaV1 in adult lobsters. KEY WORDS: Panulirus argus Virus 1 · Frozen lobster tails · Caribbean Sea Full text in pdf format PreviousNextCite this article as: Huchin-Mian JP, Briones-Fourzán P, Simá-Álvarez R, Cruz-Quintana Y and others (2009) Detection of Panulirus argus Virus 1 (PaV1) in exported frozen tails of subadult-adult Caribbean spiny lobsters Panulirus argus. Dis Aquat Org 86:159-162. https://doi.org/10.3354/dao02117 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 86, No. 2. Online publication date: September 23, 2009 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2009 Inter-Research.