In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme L Extract on human lung cancer cell line NCI-H157
2012; Volume: 32; Issue: 3 Linguagem: Inglês
10.1016/s0254-6272(13)60046-1
AutoresXiaoni Liu, Yujie Li, Qing Yang, Ying Chen, Xiaogang Weng, Yiwei Wang, Ning Li, Xiaoxin Zhu,
Tópico(s)Marine Sponges and Natural Products
ResumoTo investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract (ESC) in vitro. ESC was first extracted with ethanol, and then washed using a polyamide column with 60% ethanol. ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions. Subsequently, the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined. The results showed that ESC was rich in isomers of Chamaejasminor, neochamaejasmine and Sikokianin. ESC had significant cytotoxicity against NCI-H157 cells, with an IC50 of approximately 18.50 μg · mL−1. ESC caused significant increase in total apoptotic rate, the activity of caspase 3 and 8, and Fas protein expression (P<0.05). The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway.
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