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HPLC and heart cutting techniques of polychlorinated dibenzofurans and polychlorinated dibenzodioxins

1986; Elsevier BV; Volume: 15; Issue: 9-12 Linguagem: Inglês

10.1016/0045-6535(86)90381-4

1879-1298

M. Swerev, K. Ballschmiter,

Mass Spectrometry Techniques and Applications

A clean-up step prior to the determination of polychlorodibenzo-p-dioxins (PCDDs) and polychlorodibenzofurans (PCDFs) based on normal- phase HPLC on a 2-(1-pyrenyl)ethyldimethyl silylated silica column is presented. With hexane as mobile phase, polychlorobiphenyls (including highly chlorinated non-ortho isomers) and organochlorine pesticides are eluted in the first fraction. The total fraction of PCDDs and PCDFs can be eluted by backflushing. By application of a gradient, all congeners are eluted within 75 min. The retention order of the PCDDs and PCDFs is predominantly governed by the degree of chlorination, but the substitution pattern also has a strong influence. Subtractions of the PCDD/PCDF fraction can be taken. The fraction containing 2,3,7,8-tetrachlorodibenzo-p-dioxin was isolated. The three tetrachlorodibenzo- p-dioxin isomers of this fraction were baseline separated on a non-polar capillary GC column.