Isolation, purification and partial characterization of viper venom inhibiting factor from the root extract of the Indian medicinal plant sarsaparilla (Hemidesmus indicus R.Br.)
1994; Elsevier BV; Volume: 32; Issue: 12 Linguagem: Inglês
10.1016/0041-0101(94)90314-x
ISSN1879-3150
AutoresIqbal Alam, Biswajit Auddy, A. Gomes,
Tópico(s)Toxin Mechanisms and Immunotoxins
ResumoAn organic acid, isolated and purified from the root extract of an Indian medicinal plant sarsaparilla Hemidesmus indicus R.Br, possessed viper venom inhibitory activity. The compound (designated HI-RVIF) was isolated by solvent extraction, silica gel column chromatography and thin layer chromatography, and was homogeneous in nature. The white needle-shaped crystals were soluble in water, methanol and chloroform and had a melting point of 155–158°C and λmax 260 nm. spectral analysis confirmed the presence of a benzene ring, methoxy group, and hydroxyl group; the mol. wt of the compound was 168. HI-RVIF significantly antagonized viper venom-induced lethal, haemorrhagic, coagulant and anticoagulant activity in experimental rodents.
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