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Bioconversion of arachidonic acid to prostaglandins by graafian follicles and stroma from the human ovary

1981; Elsevier BV; Volume: 7; Issue: 6 Linguagem: Inglês

10.1016/0161-4630(81)90042-2

1878-0636

Kazuo Satoh, Naoki Mitsuhashi, Yasuo Kawai, Katsuyuki Kinoshita, Shoichi Sakamoto,

Protein Hydrolysis and Bioactive Peptides

In order to study production of prostaglandins (PGs) by human pre-ovulatory ovarian tissue, bioconversion of 14Carachidonic acid to PGs was examined using homogenates of human Graafian follicles and stromal tissues fully stimulated by endogenous follicle-stimulating hormone in the regular menstrual cycle. Among the radioactive metabolites extracted, PGE2 and 6-keto PGF1α were purified and identified by silicic acid column-, thin layer-, reversed phase partition chromatographies and radiogaschromatography. The follicular tissue was shown to convert14 C-arachidonic acid to PGE2 at a conversion rate of 0.34% but not to 6-keto PGF1α. On the other hand, the stromal tissue was demonstrated to produce not only 14C-labelled PGE2 at a conversion of 0.09% but mainly 14C-labelled 6-keto PGF1α at 0.26%.