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First Report of Dasheen mosaic virus Infecting Lotus ( Nelumbo nucifera ) in China

2015; American Phytopathological Society; Volume: 99; Issue: 10 Linguagem: Inglês

10.1094/pdis-01-15-0106-pdn

1943-7692

Yu Xia, Jiajing Sheng, Xuan Zheng, Ying Diao, Xingfei Zheng, Keqin Xie, Mingyue Zhou, Zhongli Hu,

GABA and Rice Research

HomePlant DiseaseVol. 99, No. 10First Report of Dasheen mosaic virus Infecting Lotus (Nelumbo nucifera) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Dasheen mosaic virus Infecting Lotus (Nelumbo nucifera) in ChinaX. Yu, J. J. Sheng, X. W. Zheng, Y. Diao, X. F. Zheng, K. Q. Xie, M. Q. Zhou, and Z. L. HuX. YuSearch for more papers by this author, J. J. ShengSearch for more papers by this author, X. W. ZhengSearch for more papers by this author, Y. DiaoSearch for more papers by this author, X. F. ZhengSearch for more papers by this author, K. Q. XieSearch for more papers by this author, M. Q. ZhouSearch for more papers by this author, and Z. L. HuSearch for more papers by this authorAffiliationsAuthors and Affiliations X. Yu J. J. Sheng , State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P. R. China X. W. Zheng , Guangchang White Lotus Research Institute, Guangchang, Jiangxi, 344900, China Y. Diao X. F. Zheng , State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P. R. China K. Q. Xie , Guangchang White Lotus Research Institute, Guangchang, Jiangxi, 344900, China M. Q. Zhou , Lotus Center Wuhan University, Wuhan 430072, China Z. L. Hu , State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Luojia Hill, Wuhan, Hubei, 430072, P. R. China. This research was supported by National Key Technologies R&D Program (No. 2012BAD27B01). Published Online:28 Jul 2015https://doi.org/10.1094/PDIS-01-15-0106-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Nelumbo nucifera (lotus) is a perennial aquatic plant belonging to the family Nelumbonaceae. In China, lotus is cultivated as vegetable, ornamental plant, or herbal medicine with a total land area of approximately 1 × 106 ha. Viral disease in lotus is reportedly caused by Cucumber mosaic virus (CMV), which results in stunted growth and malformation in infected plants (Ding et al. 2011). Dasheen mosaic virus (DsMV), which belongs to the genus Potyvirus in the family Potyviridae, mainly attacks various cultivated aroids and can be transmitted by aphid species (Elliott et al. 1997; Zettler et al. 1987). In February 2014, an unknown pale-green mosaic symptom was observed in the leaves of lotus grown at the Lotus Germplasm Repository of Wuhan University. Severely infected lotus leaves eventually curled and shrank, and the growth of rhizomes was also affected by this unknown disease. In the field, the incidence of this disease in lotus was as high as 8%. To identify the pathogen of this disease, 30 infected lotus samples were collected for analysis with double-antibody sandwich (DAS)-ELISA kit and reverse transcription (RT)-PCR. First, infected lotus leaves showing pale green mosaic symptom were analyzed using DsMV-ELISA kit (Dongge Co. LTD) in accordance with the manufacturer’s protocol, and the healthy lotus leaves were used as negative control. Absorbance at 450 nm was read in ELISA reader within 15 min after adding stop solution. Results showed that the infected lotus samples were positive to DsMV. These DsMV-positive samples were further analyzed by RT-PCR using a pair of specific primers (forward, 5′-GGGCTTGGGTGATGATGGA-3′; reverse, 5′-GCCTTTCAGTGTTCTCGCTTG-3′) designed on the basis of core nucleotide sequences of the DsMV cp gene. As expected, the amplified PCR product was 357 bp in size, and this product was sequenced and then this sequence was deposited in GenBank (Accession No. KP692755). BLAST results showed high sequence similarity ranging from 84 to 95% between the tested sample and other published DsMV isolates. The virus isolated from DsMV-positive samples was mechanically transmitted to the healthy lotus. After 10 days, a pale green mosaic symptom appeared in the leaves. ELISA and RT-PCR results showed that DsMV was positive and CMV was negative in these artificially infected lotus samples. Both serologic and molecular test results confirmed that DsMV is the pathogen that caused the disease in lotus. This case provides the first report of DsMV infection in lotus in China. To our knowledge, CMV was the only pathogen reported to cause viral disease in lotus. In this study, we identified DsMV as a new pathogen causing viral disease in lotus. Our results will be highly valuable for disease prevention and control in lotus cultivation.References:Ding, Z. L., et al. 2011. Hubei Agric. Sci. 50:3078. Google ScholarElliott, M. S., et al. 1997. Plant Pathol. Circ. No. 38. Fla. Dept. Agric., Tallahassee. Google ScholarZettler, F. W., and Hartman, R. D. 1987. Plant Dis. 71:958. https://doi.org/10.1094/PD-71-0958 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 99, No. 10 October 2015SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 5 Oct 2015Published: 28 Jul 2015First Look: 14 Apr 2015Accepted: 7 Apr 2015 Page: 1449 Information© 2015 The American Phytopathological SocietyCited byDasheen mosaic virus (dasheen mosaic)CABI Compendium, Vol. CABI CompendiumAntiserum Preparation of Recombinant Sweet Potato Latent Virus-Lotus (SPLV-Lotus) Coat Protein and Application for Virus-Infected Lotus Plant DetectionThe Plant Pathology Journal, Vol. 36, No. 6Genetic Variation of the Novel Badnaviruses Infecting Nelumbo Nucifera Based on the RT/RNase H Coding Region SequencesHorticultural Plant Journal, Vol. 6, No. 5Genomic and biological characterization of a novel strain of sweet potato latent virus isolated from lotus (Nelumbo nucifera Gaertn.)8 June 2019 | Journal of Plant Pathology, Vol. 101, No. 4Nelumbo nucifera (Indian lotus)6 June 2020Molecular Characterization and Distribution of Two Strains of Dasheen mosaic virus on Taro in HawaiiYanan Wang, Beilei Wu, Wayne B. 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