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Inhibitory Effect of Mao-Bushi-Saishin-to on Prostaglandin E2 Synthesis in C6 Rat Glioma Cells

2004; Pharmaceutical Society of Japan; Volume: 27; Issue: 7 Linguagem: Inglês

10.1248/bpb.27.1133

1347-5215

Shinya Saito, Sachie Kamiyama, Mayumi Oda, Norimichi Nakahata, Yasushi Ohizumi,

Natural product bioactivities and synthesis

Effect of Mao-Bushi-Saishin-to (Ma-Huang-Fu-Zi-Xi-Xin-Tang: MBS) on prostaglandin E2 (PGE2) production was investigated using C6 rat glioma cells. Mao or Saishin inhibited histamine-induced PGE2 production while MBS slightly decreased and Bushi increased it. MBS and Mao inhibited and Bushi enhanced A23187-induced PGE2 production while Saishin had no effect. Concomitantly, Mao inhibted, but Bushi fascilitated, histamine- and A23187-induced phosphorylation of extracellular signal-regulated kinase (ERK)1/2. Treatment of MBS, Mao and also Saishin increased cAMP content. From these results, MBS inhibit PGE2 production in C6 cells, mainly due to Mao but also due to Saishin at least in part, and the counteraction of Bushi. The former effect is mediated by formation of cAMP and resulting inhibition of ERK1/2-phosphorylation.