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Galanin inhibits rat pancreatic amylase release via cholinergic suppression

1992; Elsevier BV; Volume: 13; Issue: 3 Linguagem: Inglês

10.1016/0196-9781(92)90079-i

1873-5169

Kenneth M. Flowe, Kathleen M. Lally, Michael W. Mulholland,

Diabetes Treatment and Management

The effects of galanin on pancreatic exocrine function were examined using rat pancreatic tissues. In anesthetized rats, galanin (40 μg/kg/h) decreased amylase secretion stimulated by 2-deoxy glucose (5.8 ± 0.1 vs. 3.1 ± 0.1 times basal) and cholecystokinin octapeptide (21.5 ± 0.6 vs. 16.8 ± 0.5), while not inhibiting bethanechol-stimulated secretion. In dispersed acini, there was no effect of galanin alone (10−8 to 10−13 M) on amylase release, nor did galanin (10−6 or 10−8 M) coincubation affect amylase release stimulated by bethanechol (10−3 to 10−7 M) or CCK-8 (10−8 to 10−13 M). Using pancreatic lobules, coincubation with galanin (10−6 M) suppressed 75 mM KCl-stimulated amylase secretion and ACh release (10.1 ± 0.6% vs. 7.3 ± 0.4%). Veratridine-stimulated (10−4 M) amylase secretion and ACh release (12.4 ± 1.7% vs. 8.5 ± 0.7%) were similarly diminished.