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Solubilized elastin as a substrate for elastase and elastase inhibitor determinations

1971; Academic Press; Volume: 5; Issue: 4 Linguagem: Inglês

10.1016/0006-2944(71)90038-x

1557-7996

Stephen Keller, Ines Mandl,

ATP Synthase and ATPases Research

This paper describes a test for elastase which employs oxalic acid-solubilized elastin as the substrate. The test described is more sensitive than those using insoluble elastin-dye complexes and more specific than the recently introduced tests using synthetic peptide-esters. It can be used in the presence of other proteins, including serum, and is especially suited for the study of serum elastase inhibitors in health and disease.